Expression of miR-338-3p and PLCD3 in papillary thyroid carcinoma tissues and association with pathological pa-rameters,epithelial-mesenchymal transition and prognosis
Objective To investigate the expression of micro ribonucleic acid-338-3p(miR-338-3p)and phospho-lipase C delta-3(PLCD3)in papillary thyroid carcinoma(PTC)tissues and their relationship with pathological parameters,epi-thelial-mesenchymal transition(EMT)and prognosis.Methods 152 cases of PTC patients admitted to the Breast and Thy-roid Surgery Department of Shangluo Central Hospital who underwent surgical resection from January 2016 to December 2020 were selected,and real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of miR-338-3p and PLCD3 mRNA in cancer tissues and corresponding para-cancerous tissues,and immunohistochemistry was used to detect the expression of EMT-associated proteins[N-calmodulin(N-Cad),E-calmodulin(E-Cad),and vimentin(VIM)].The relationship between miR-338-3p,PLCD3 mRNA expression and pathological parameters of PTC were analyzed.The binding sites of miR-338-3p and PLCD3 were predicted by Targetscan database,and the correlation between mriR-338-3p and PLCD3 mRNA and the expression of both with EMT-related proteins in PTC tissues was analyzed by Pearson's method and dichotomous correlation.PTC tissues were divided into high/low miR-338-3p,PLCD3 mRNA expression groups accord-ing to the mean values of miR-338-3p,PLCD3 mRNA expression,and the survival curves of disease-free survival(DFS)of PTC patients with high/low miR-338-3p,PLCD3 mRNA expression were plotted by Kaplan-Meier method.Factors of DFS in PTC patients were analyzed by Cox regression.Results Compared with para-cancerous tissues,miR-338-3p expression and E-Cad protein positive expression rate were decreased in PTC tissues,and PLCD3 mRNA expression and N-Cad and VIM protein positive expression rate were increased(t/x2/P=32.875/＜0.001,15.575/＜0.001,37351/＜0.001,21.984/＜0.001,16.604/＜0.001).miR-338-3p was negatively correlated with PLCD3 mRNA expression in PTC tissues(r/P=-0.712/＜0.001).miR-338-3p was negatively correlated with the positive expression rate of N-Cad and VIM proteins,and positively correlated with that of E-Cad proteins in PTC tissues(r/P=-0.642/＜0.001,-0.617/＜0.001,0.622/＜0.001);PLCD3 mRNA was negatively correlated with N-Cad in PTC tissues,VIM protein positive expression rate was positively correlated with E-Cad protein pos-itive expression rate(r/P=0.657/＜0.001,0.624/＜0.001,-0.632/＜0.001).Comparison of miR-338-3p and PLCD3 mRNA ex-pression in PTC tissues with different TNM stages and lymph node metastasis showed differences(F/t/P=30.778/＜0.001,3.430/0.001).3-year DFS was higher in the miR-338-3p high-express ion group than that in the miR-338-3p low-expression group,and 3-year DFS was lower in the PLCD3 mRNA high-expression group than that in the PLCD3 mRNA low-expression group(x2/P=15.615/＜0.001,16.088/＜0.001).TNM stage Ⅲ,lymph node metastasis,and PLCD3 mRNA ≥ 1.83 were inde-pendent risk factors for DFS in PTC patients,and miR-338-3p ≥0.57 was an independent protective factor[HR(95％CI)=3.127(1361-7.604),2-46(1.115-5.817),2.854(1.178-6.919),0.306(0.116-0.804)].Conclusion Low expression of miR-338-3p and high expression of PLCD3 mRNA in PTC tissues are associated with TNM staging,lymph node metastasis,EMT and prognosis,and may become a new target for PTC diagnosis and treatment.
Papillary thyroid carcinomaMicro ribonucleic acid-338-3pPhospholipase C delta 3Pathological pa-rametersEpithelial-mesenchymal transitionPrognosis
维普
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