Objective To analyze the expression and clinical significance of microribonucleic acid-143-5p(miR-143-5p)and microribonucleic acid-335-5p(miR-335-5p)in cervical cancer tissues.Methods From July 2018 to June 2020,120 cervical cancer patients treated in our hospital were collected.Due to 5 cases of lost follow-up,a total of 115 cases were in-cluded in the statistical calculation;real-time fluorescence quantitative PCR(RT-qPCR)method was applied to detect the rel-ative expression levels of miR-143-5p and miR-335-5p in cervical cancer tissue and its adjacent tissues;multivariate Logistic regression was applied to analyze the factors affecting the prognosis of cervical cancer patients;receiver operating charac-teristic(ROC)curve was applied to analyze the value of miR-143-5p combined miR-335-5p in predicting the prognosis of cer-vical cancer patients.Results The levels of miR-143-5p and miR-335-5p in cancer tissues were obviously lower than those in adjacent tissues(t/P=22.710/＜0.001,22.950/＜0.001);the levels of miR-143-5p and miR-335-5p in cervical cancer tissues of patients with FIGO staging of stages Ⅲ-Ⅳ,poorly differentiated tumors,and lymph node metastasis were obviously lower than those of patients with FIGO staging of stages Ⅰ-Ⅱ,highly differentiated tumors,and no lymph node metasta-sis(miR-143-5p:x2/P=3.041/0.003,3.573/0.001,3.443/0.001;miR-335-5p:x2/P=5.769/＜0.001,2.890/0.005,2.738/0.007);the lev-els of miR-143-5p and miR-335-5p in cancer tissues of the poor prognosis group were obviously lower than those of the good prognosis group(t/P=3.718/＜0.001,3.150/0.002);the proportion of patients with FIGO stage Ⅰ-Ⅱ and no lymph node metastasis in the good prognosis group was higher than that in the poor prognosis group(x2/P=18.261/＜0.001,9.635/0.002);FIGO stage Ⅲ-Ⅳ was risk factor for cervical cancer patients[OR(95％CI)=4.105(1.484-11.353)],while high levels of miR-143-5p and miR-335-5p were protetive factors[OR(95％CI)=0.196(0.084-0.458),0.220(0.088-0.549)];the AUC of serum miR-143-5p,miR-335-5p and their combination in predicting the prognosis of patients with cervical cancer were 0.743,0.726 and 0.879,respectively,and the combination of the two was better than their single prediction value(Z/P=2.346/0.019,2.601/0.009).Conclusion MiR-143-5p and miR-335-5p are downregulated in cervical cancer tissue,and the com-bination of the two has a high value in predicting the prognosis of cervical cancer patients.
维普
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