Objective To investigate the changes in s erum levels of microRNA-335-5p(miR-335-5p)and microR-NA-141-3p(miR-141-3p)in patients with polycystic ovary syndrome(PCOS)and their diagnostic value.Methods A total of 145 patients with PCOS treated in the Gynecology Department of Nantong Hospital of Traditional Chinese Medicine from February 2019 to June 2022 were selected as PCOS group and divided into IR subgroup and non-IR subgroup according to HOMA-IR.They were divided into obese subgroup and non-obese subgroup according to BMI.According to the level of testosterone,they were divided into high androgen subgroup and non-high androgen subgroup.Another 110 female health examination subjects in the same period were selected as control group.The serum miR-335-5p and miR-141-3p levels of the two groups/different clinical conditions were detected and compared by fluorescence quantitative PCR.Pearson correlation or Spearman rank correlation coefficient described the correlation between serum miR-335-5p and miR-141-3p and clinical in-dicators.Multi factor logistic was applied to analyze the factors that affected the occurrence of PCOS.ROC curve was ap-plied to analyze the diagnostic value of serum miR-335-5p and miR-141-3p levels for PCOS.Results By comparison of clini-cal data,the levels of BMI,FINS,LH,testosterone(T)and HOMA-IR in PCOS group were significantly higher than those in control group(t/P=4.881/＜0.001,16326/＜0.001,37.901/＜0.001,43.690/＜0.001,15.395/＜0.001).Compared with control group,serummiR-335-5p and miR-141-3p levels in PCOS group were decreased(t/P=10.516/＜0.001,8.767/＜0.001).The lev-els of miR-335-5p and miR-141-3p in IR subgroup,obesity subgroup and hyperandrogenism subgroup were lower than those in non-IR subgroup,non-obesity subgroup and non-hyperandrogenism subgroup(t/P=2.882/0.005,3.783/＜0.001,5.196/＜0.001,4.515/＜0.001,5.069/＜0.001,4.296/＜0.001);Correlation analysis showed that serum miR-335-5p,miR-141-3p levels were negatively correlated with IR,BMI,testosterone(P＜0.01).Serum miR-335-5p,miR-141-3p,BMI,Fins,LH,testoster-one and HOMA-IR were all risk factors for PCOS[OR(95％CI)=1.812(1.348-2.436)、2.536(1.647-3.842)、2.913(1.523-5 573)、1293(1207-1385)、1.692(1.180-2.427)、3.452(2518-4.733)、4.620(1.916-11.139)];The area under the curve(AUC)of serum miR-335-5p,miR-141-3p and their combination in the diagnosis of PCOS patients were 0.818,0.733 and 0.873.The com-bined detection of miR-335-5p and miR-141-3p had more diagnostic efficacy than that of serum miR-335-5p and miR-141-3p alone(Z=2.882,4.767,P＜0.001).Conclusion Serum miR-335-5p and miR-141-3p are low expressed in patients with PCOS,they may be involved in IR,obesity,and hyperandrogenism secretion in PCOS patients.The combined diagnosis of the two has more diagnostic efficacy for PCOS and is expected to become an important method for assisting in the diagnosis of PCOS.
Polycystic ovary syndromeMicro RNA-335-5pMicro RNA-141-3pDiagnostic value
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万方数据
