摘要
体液活检与传统侵入性检查相比具有许多独特的优势,尤其在产前诊断、器官移植后免疫排异反应、肿瘤早期诊断和肝脏疾病诊疗方面展现出巨大潜力.在肝脏疾病领域,游离DNA参与机体内在的炎性反应和凝血过程.基于表观遗传学的研究,游离DNA片段的甲基化分析对肝脏恶性肿瘤的早期诊断和治疗大有帮助.在器官移植后,游离DNA有望成为监测免疫排异反应的重要标志物,为关于免疫排异反应机制的研究提供了新角度.文章对游离DNA的来源、提取方法、检测方法,以及其在肝移植、肝癌、肝衰竭等疾病中的研究应用进展进行综述.
Abstract
Body fluid biopsy exhibits significant promise in prenatal diagnostics,post-organ transplantation immune rejection monitoring,early tumor detection,and liver diseases due to its distinct advantages over conventional invasive ex-aminations.In the realm of liver diseases,circulating-free DNA not only mirrors hepatic function but potentially participates in the pathophysiological cascades of inflammation and coagulation alterations.The unique fragments within free DNA,cou-pled with methylation analysis,contribute to the early diagnosis of tumors and provide guidance for treatment strategies.Following organ transplantation,circulating-free DNA emerges as a pivotal marker for immune rejection reaction surveillance,offering a novel vantage point for scrutinizing rejection mechanisms.This article reviews the sources,extraction methods,and detection methods of free DNA,and the progress of its research application in liver transplantation,hepatocellular carci-noma,and liver failure.
基金项目
云南省科技厅科技重大专项(202302AA310018)
"春城计划"高层次创新创业团队专项(2022SCP002)
维普
万方数据