Objective To explore the relationship between serum miR-183-3 p and miR-495-3p expression and clini-cal pathological characteristics and prognosis of esophageal cancer patients.Methods A total of 105 patients with esopha-geal cancer admitted to The Third Medical Center of the General Hospital of the People's Liberation Army from December 2017 to December 2020 as the esophageal cancer group,and another 105 healthy individuals who underwent physical exami-nations as the control group.The qRT-PCR method was applied to detect the expression of serum miR-183-3p and miR-495-3p.Kaplan-Meier curve was plotted to analyze the relationship between miR-183-3p,miR-495-3p and the prognosis of esophageal patients.ROC was applied to analyze the predictive value of miR-183-3p and miR-495-3p for the prognosis of esophageal cancer patients.COX risk regression model was applied to analyze the factors affecting the prognosis of esoph-ageal cancer patients.Results The expression level of miR-183-3p in the esophageal cancer group was higher than that in the control group,while the expression level of miR-495-3p was lower than that in the control group(t=8.760,4.054,P＜0.001).The expression of serum miR-183-3p in the death group was higher than that in the survival group,while the expres-sion of serum miR-495-3p was lower than that in the survival group(t=3.655,4210,P＜0.001).Patients with high expression of miR-183-3p had an increased degree of low differentiation and the proportion of clinical stage Ⅲ-Ⅳ of AJCC,while pa-tients with low expression of miR-183-3p had an increased degree of middle and high differentiation and the proportion of clinical stage Ⅰ-Ⅱ of AJCC,and the differences were statistically significant(x2=10.680,P=0.001;x2=4.246,P=0.001).Patients with high expression of miR-495-3p had an increased degree of middle and high differentiation and the proportion of clinical stage Ⅰ-Ⅱ of AJCC,while patients with low expression of miR-495-3p had an increased degree of low differentia-tion and the proportion of clinical stage Ⅲ-Ⅳ of AJCC,and the differences were statistically significant(x2=5.341,P=0.021;x2=9320,P=0.002).The 3-year survival rate of patients with high expression of miR-183-3p(67.92％)was lower than that of patients with low expression of miR-183-3p(84.62％)(x2=5.279,P=0.022),and the 3-year survival rate of patients with low expression of miR-495-3p(64.81％)was lower than that of patients with high expression of miR-495-3p(8824％)(x2=9351,P=0.002).The AUC of serum miR-183-3p,miR-495-3p and mir-495-3p in predicting prognosis of patients with esophageal cancer were 0.763,0.737 and 0.864,respectively,and the AUC of combined was superior to that of their individu-al predictive efficacy(Z=2.564,2.011,P=0.010,0.043).Low differentiation,AJCC clinical stage Ⅲ-Ⅳ,high expression of miR-183-3p,and low expression of miR-495-3p were risk factors affecting the prognosis of esophageal cancer patients[HR(95％CI)=6.628(2278-19288),5.803(2261-14.897),5.130(2.187-12.034),6.152(1.951-19.401)].Conclusion Serum miR-183-3p is highly expressed and miR-495-3p is low in esophageal cancer patients.The expression of miR-183-3p and miR-495-3p is related to the degree of tumor differentiation and clinical stage of AJCC in patients,and can be used as biological indi-cators to predict prognosis.
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