Objective To study and analyze the relationship between serum long non-coding RNA KCNQ1 oppo-site strand/antisense transcript 1(LncRNA KCNQ1OT1),microribonucleic acid-192-5p(miR-192-5p)and short-term progres-sion of albuminuria and prognosis in patients with diabetic nephropathy.Methods A total of 102 patients with diabetic ne-phropathy admitted to Department of Endocrine Rheumatology and Immunology,Qingdao Central Hospital,University of Health and Rehabilitation Sciences(Qingdao Central Hospital)from April 2021 to December 2022 were regarded as the dis-ease group.according to the 24 h urine albumin level at time,they were separated into a non-progressing subgroup(n=78)and a progressing subgroup(n=24).According to the prognosis,they were separated into a good prognosis group(n=84)and a poor prognosis group(n=18).58 healthy volunteers who underwent physical examination were regarded as the con-trol group.QRT-PCR was applied to detect the relative expression levels of serum LncRNA KCNQ1OT1 and miR-192-5p.General clinical data of patients were collected and analyzed.Multivariate logistic regression was applied to analyze the influ-encing factors of short-term progression of albuminuria in patients.Receiver operating characteristic(ROC)curve was plot-ted to analyze the predictive value of serumLncRNA KCNQ1OT1,miR-192-5p on prognosis.Pearson method was applied to analyze the correlation between LncRNA KCNQ1OT1 and miR-192-5p.Results Compared with the control group,the ser-um LncRNA KCNQ1OT1 level in the diseased group increased,while the miR-192-5p level decreased(t/P=17.619/＜0.001,16.120/＜0.001).Compared with the non-progression group,the serum LncRNA KCNQ1OT1 level in the progression group increased,while the miR-192-5p level decreased(t/P=7.698/＜0.001,9.485/＜0.001).There was a statistically obvious differ-ence in blood urea nitrogen(BUN),creatinine(Scr),fasting blood glucose(FBG),and glycated hemoglobin(HbA1c)between the two groups(x2/P=9.835/0.002,t/P=4.582/＜0.001,3.139/0.002,2.593/0.011,2.356/0.020).Compared with good prognosis group,poor prognosis group showed an increase in serum LncRNA KCNQ1OT1 level and a decrease in miR-192-5p level(t/P=7.602/＜0.001,9.380/＜0.001).LncRNA KCNQ1OT1 is negatively correlated with miR-192-5p(r/P=-0.452/＜0.001).Hy-pertension and elevated serum LncRNA KCNQ1OT1 levels were risk factors for short-term progression of albuminuria in pa-tients[OR(95％CI)=1.532(1.058-2219);1.638(1.100-2.438)],while elevated miR-192-5p level was a protective factor for short-term progression of albuminuria in patients[OR(95％CI)=0.671(0.517-0.871)].The AUC of LncRNA KCNQ1OT1,miR-192-5p,and their combined prediction for patient prognosis was 0.808,0.822,and 0.896,respectively.The combined pre-diction was obviously better than individual diagnosis of LncRNA KCNQ1OT1 and miR-192-5p(Z/P=2.030/0.042,2.116/0.034).Conclusion The serum LncRNA KCNQ1OT1 level in patients with diabetic nephropathy is increased,and the miR-192-5p level is decreased.Both are factors influencing the short-term progression of albuminuria in patients with diabetic ne-phropathy,and have a certain auxiliary predictive value for the prognosis of patients.
Diabetic nephropathyLncRNA KCNQ1OT1Microribonucleic acid-192-5pShort term progression of albuminuriaPrognosis
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