Objective To investigate the expression of long non-coding RNA zinc finger E-box binding homeobox protein 1 antisense chain 1(LncRNA ZEB1-AS1)and long non-coding RNA SOX2 overlapping transcript(LncRNA SOX2OT)in patients with diabetic nephropathy(DN)and their correlation with renal function.Methods In this study,106 patients with DN admitted in Department of Nephrology,Renmin Hospital of Wuhan University from November 2021 to De-cember 2023 were collected as the DN group,and 106 patients with simple diabetes in the hospital were regarded as the con-trol group.Both groups were collected venous blood.The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT were detected.According to the renal function of DN patients,they were separated into groups.The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT,and renal function,blood lipids,and blood glucose levels were compared.Pearson meth-od was applied to analyze the correlation between LncRNA ZEB1-AS1 and LncRNA SOX2OT with renal function indicators.Logistic was applied to analyze the factors affecting renal function impairment in DN patients.Results The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT in the DN group were lower than those in the control group(t=11.471,10257/all P＜0.001).The levels of serum LncRNA ZEB1-AS1 and LncRNA SOX2OT in the normal urine protein subgroup were higher than those in the microalbuminuria subgroup and higher than those in the large urine protein subgroup(F=58.720,117.722,122.493,595.589,53.178/all P＜0.001).The levels of serum BUN,SCr,UA in the normal urine protein subgroup were lower than those in the microalbuminuria subgroup and lower than those in the large urine protein subgroup(F=122.493,595589,53.178,all P＜0.001).LncRNA ZEB1-AS1 was negatively correlated with BUN,SCr,UA(r=-0.487,-0.498,-0.521,P＜0.001),there was a negative correlation between LncRNA SOX2OT and the above indicators(r=-0.527,-0.515,-0.534,P＜0.001).Logistic model analysis showed that the course of diabetes,BUN,SCr and UA levels were risk factors for renal function damage in DN patients[OR(95％CI)=1.672(1.128-2.479),2.839(1.534-5.253),2.754(1.512-5.017),2.693(1.464-4.954)],and high LncRNA ZEB1-AS1 and high LncRNA SOX2OT were protective factors[OR(95％CI)=0.875(0.798-0.959),0.898(0.832-0.969)].Conclusion Serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT are re-lated to renal function in DN patients,and may be potential indicators for evaluating renal function in DN patients.
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