Objective To analyze the expression of miR-210 and miR-143 in serum of children with bronchial asth-ma and their relationship with airway inflammation.Methods A total of 186 children with bronchial asthma admitted to De-partment of Pediatrics Langfang City People's Hospital from January 2020 to July 2022 were selected as Bronchial asthma group,100 healthy children who underwent physical examinations during the same period were collected as Healthy control group,qRT-PCR method was applied to detect serum levels of miR-210 and miR-143,Pearson method was applied to analyze the correlation between miR-210,miR-143 levels and TNF-α,IL-4,IL-6,EOS,FeNO,IL-10,IL-12,IgE,IFN-γ in children with bronchial asthma;Logistic regression was applied to analyze the influencing factors of bronchial asthma in children.Results The serum miR-210 level in children with bronchial asthma were obviously higher than those in healthy children,and the miR-143 level was obviously lower than those in healthy children(t=8.317,9.545,P＜0.001),the serum miR-210 level in chil-dren with mild,moderate,and severe asthma increased sequentially,while the miR-143 level decreased sequentially(F=7.566,19.914,P＜0.001).The levels of FVC,FEV 1,VE,PEF,IL-10,IL-12,IgE,IFN-γ in children with bronchial asthma were obviously lower than those in healthy children,while the levels of Raw,IL-4,IL-6,TNF-α,EOS,and FeNO were obviously higher than those in healthy children(P＜0.05).The serum levels of miR-210 and miR-143 in children with bronchial asthma were related to allergies,respiratory infections,and coughing(P＜0.05).Pearson analysis showed there was a negative cor-relation between serum miR-210 and miR-143 levels(r=-0.362,P＜0.001),the serum miR-210 level was positively correlated with TNF-α,IL-4,IL-6,EOS,and FeNO(r=0326,0.359,0.315,0.334,0312,P＜0.001),while negatively correlated with IL-10,IL-12,IgE,and IFN-γ(r=-0.406,-0303,-0317,-0.453,P＜0.001);the level of miR-143 was negatively correlated with TNF-α,IL-4,IL-6,EOS,and FeNO(r=-0.336,-0324,-0.323,-0.424,-0.397,P＜0.001),but positively correlated with IL-10,IL-12,IgE,and IFN-γ(r=0.338,0338,0327,0.467,P＜0.001);miR-210,miR-143,TNF-α,IL-4,IL-6,EOS,FeNO,IL-10,IL-12,IgE,and IFN-γ were all influencing factors for the occurrence of bronchial asthma in children[OR(95％CI)=1.025(1.015-1.035),0.857(0.788-0.932),1.034(1.020-1.048),1.136(1.059-1219),1243(1.147-1.347),1.349(1.151-1.581),1.408(1.178-1.683),0.932(0.900-0.965),0.473(0312-0.718),0.671(0.549-0.820),0.583(0.437-0.778)].Conclusion The in ser-um miR-210 level increases and the in miR-143 level decreases in children with bronchial asthma,they are closely related to the progression of airway inflammation.
国家科技期刊平台
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万方数据
