Objective To investigate the intervention effect of microvascular endothelial cell injury induced by high glucose and high fat and the intervention effect of Shensong Yangxin Capsule(SSYX).Methods The experiments were conducted from June 2022 to February 2023 at the State Key Laboratory for Innovation and Transformation of Luobing Theory.Human microvascular endothelial cells were randomly divided into the normal group,the model group,the SSYX-L,the SSYX-M and the SSYX-H group(025,05,1.0 mg/L).In addition to the normal group,the other 4 groups were treated with high glucose and high fat to establish the human microvascular endothelial cell injury model,and carried out corresponding intervention.ELIS A was used to detect cell activity,fluorescence microscopy was used to detect cellular mitochondrial mem-brane potential,and Western blot was used to detect protein levels of DRP1,MFN2,GRP78,NF-κB p65,P-selectin,E-selec-tin,and IL-6.Results Compared with the normal group,the cell survival activity and mitochondrial membrane potential of the model group were significantly reduced,the protein expressions of DRP1,GRP78,NF-κB p65,P-selectin,E-selectin and IL-6 were significantly increased,and the expression of MFN2 protein was significantly decreased.Compared with the model group,the cell survival activity and mitochondrial membrane potential of each dose group were significantly increased(P＜0.01),and the protein expressions of GRP78,NF-κB p65 and E-selectin were significantly decreased(P＜0.01 or＜0.05).The expression ofMFN2 protein in the SSYX-H group was significantly increased(P＜0.05),and the expression of IL-6 protein was significantly decreased(P＜0.05),and the expression of DRP1 and P-selectin protein in the SSYX-M and SSYX-H groups was significantly decreased(P＜0.05 or＜0.01).Conclusion Shensong Yangxin Capsule can improve the survival activity of microvascular endothelial cells,maintain mitochondrial homeostasis,reduce endoplasmic reticulum stress,inhibit inflammatory response,and reduce the level of adhesion molecules,thereby protecting microvascular endothelial cells induced by high glucose and high fat.
High glucose and high fatMicrovascular endothelial cellsShensong Yangxin CapsulesMitochondriaEndoplasmic reticulum stressInflammatory reactionMechanism of action
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