Correlation of serum LncRNA-MALAT1 and LncRNA-XIST with in-stent restenosis at 1 year after stenting in patients with lower limb atherosclerotic occlusive disease
Correlation of serum LncRNA-MALAT1 and LncRNA-XIST with in-stent restenosis at 1 year after stenting in patients with lower limb atherosclerotic occlusive disease
Objective To investigate the correlation between serum long-chain non-coding RNA(LncRNA)-metas-tasis-adenocarcinoma metastasis-associated transcript 1(MALAT1),LncRNA-X inactivation specific transcript(XIST)and in-stent restenosis(ISR)within 1 year after stenting in patients with lower extremity arteriosclerosis obliterans(LEASO).Methods One hundred and forty-seven LEASO patients who underwent stent implantation in the Department of Vascular Surgery of the Affiliated Cancer Hospital of Inner Mongolia Medical University from January 2018 to December 2022 were selected as the study subjects,and they were divided into the ISR group(41 cases)and the no-ISR group(106 cases)based on the occurrence of ISR within 1 year,and the real-time fluorescence quantitative PCR was used to detect the postopera-tive serum LncRNA-MALAT1,LncRNA-XIST levels;the correlation between serum LncRNA-MALAT1 and LncRNA-XIST levels and lumen diameter reduction rate in LEASO patients was analyzed by Pearson's method,the factors of ISR within 1 year after stent implantation in LEASO patients were analyzed by multifactorial logistic regression,and ROC curves were plotted to analyze the serum LncRNA-MALAT1,The predictive value of LncRNA-XIST levels for ISR within 1 year after stenting in LEASO patients.Results At 1-year follow-up,the incidence of ISR after stenting was 27.89％(41/147)in 147 LEASO patients;serum LncRNA-MALAT1 and LncRNA-XIST levels were elevated in the ISR group compared with the no-ISR group(t/P=5.949/＜0.001,5.927/＜0.001);Pearson's correlation showed a positive correlation between serum LncRNA-MALAT1 and LncRNA-XIST levels and the rate of lumen diameter reduction in LEASO patients(r/P=0.596/＜0.001,0.588/＜0.001);Multifactorial logistic regression showed that diabetes mellitus and elevated C-reactive protein,LncRNA-MALAT1,and LncRNA-XIST were independent risk factors for ISR within 1 year of stent implantation in patients with LEASO[OR(95％CI)=3.185(1.155-8.781),1.187(1.003-1.404),1.433(1.199-1.713),1.575(1242-1.998)];The ROC curve showed that the area under the curve for the combined serum LncRNA-MALAT1 and LncRNA-XIST levels predicting ISR within 1 year after stent implantation in LEASO patients was 0.906,which was greater than that predicted by the serum Ln-cRNA-MALAT1 and LncRNA-XIST levels alone,which was 0.799 and 0.786(Z/P=3.176/0.002,3.513/＜0.001).Conclusion Elevated serum LncRNA-MALAT1 and LncRNA-XIST levels after stent implantation in LEASO patients are independent risk factors for ISR within 1 year,and the combination of serum LncRNA-MALAT1 and LncRNA-XIST levels has a high predictive value for ISR within 1 year after stent implantation in LEASO patients.
维普
万方数据