Objective To explore the relationship between the serum expression levels of long non-coding RNAPVT1 gene(LncRNAPVT1)and microRNA-486-5p(miR-486-5p)in plasma and the pathological characteristics and prog-nosis of acute myeloid leukemia(AML)patients.Methods One hundred patients with AML were selected as AML group and 100 healthy subjects were selected as healthy control group.The relative expression levels of serum LncRNA PVT1 and miR-486-5p were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Pearson correlation method was used to evaluate the correlation between serum LncRNA PVT1 and miR-486-5p in AML patients and its rela-tionship with pathological characteristics.Kaplan-Meier method was used to analyze the survival curve of AML patients,and Cox regression analysis was used to evaluate the risk factors affecting the prognosis of AML patients.Results The levels of LncRNA PVT1 in AML experimental group patients were higher than those in the healthy control group.the levels of miR-486-5p in AML experimental group patients were lower than those in the healthy control group(t/P=15.403/＜0.001、18305/＜0.001).Pearson correlation analysis showed that LncRNA PVT1 was negatively correlated with miR-486-5p level in AML group(r=-0261,P＜0.01).There were statistically significant differences in leukocyte count,bone marrow original cell ratio,prognostic risk stratification,LncRNA PVT1 and miR-486-5p levels in AML group(LncRNA PVT1:t/P=2.934/0.004,2.901/0.005,10.985/＜0.001;miR-486-5p:t/P=2.598/0.012,2.604/0.012,8.593/＜0.001).The 3-year overall survival rate of those with high expression of LncRNA PVT1 was lower than those with low expression of LncRNA PVT1(x2/P=14.160/＜0.001),and the 3-year overall survival rate of those with high expression of miR-486-5p was higher than that of those with low ex-pression of miR-486-5p(x2/P=16.820/＜0.001).Comprehensive analysis of study data showed that high level of LncRNA PVT1 expression and low level of miR-486-5p expression were risk factors for poor prognosis in patients with acute myeloid leukemia[RR(95％CI)=1.982(1.148-2.993),1.668(1.085-2.641),1.659(1.068-2.602)].Conclusion The high expres-sion of LncRNA PVT1 and low expression of miR-486-5p in AML patients are related to white blood cell count,bone mar-row primitive cell ratio,risk stratification of prognosis and patient prognosis.
维普
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