Objective To investigate the expression and prognostic value of serum long chain non coding RNA nuclear enriched transcript 1(LncRNA NEAT1)and trefoil factor 1(TFF1)in children with retinoblastoma(Rb).Methods Eighty-five Rb patients who underwent ophthalmic surgery at Ophthalmology Department of Beijing Anzhen Hospital Affili-ated to Capital Medical University Nanchong Hospital from January 2018 to January 2021 were selected as the Rb group,while 60 healthy individuals who underwent physical examinations during the same period were selected as the healthy con-trol group.Real time fluorescence quantitative PCR was used to detect serum LncRNA NEAT1 levels,and enzyme-linked im-munosorbent assay was used to detect serum TFF1 levels;the relationship between serum LncRNA NEAT1,TFF1 and clini-cal pathological characteristics was analyzed;the evaluation value of serum LncRNA NEAT1 and TFF1 in Rb patients was analyzed by job characteristic curve of subjects.Results The serum LncRNA NEAT1 in the Rb group was(3.12±0.43)higher than that in the healthy control group(0.82±0.21),the serum TFF1 of the Rb group was(22.14±4.61)μ g/L,which was lower than that of the healthy control group(64.01±9.63)μg/L(t/P=38.305/＜0.001,34.858/＜0.001).Compared with patients with tumor diameter＜20 mm and pathologically differentiated Rb,patients with tumor diameter ≥ 20 mm and undifferentiated Rb had higher serum LncRNA NEAT1 and lower TFF1(Lnc RNANEAT1:t/P=17.925/＜0.001,16.848/＜0.001;TFF1:t/P=12.505/＜0.001,8.120/＜0.001).The 3-year progression free survival rate of the high expression group of se-rum LncRNA NEAT1 was lower than that of the low expression group(57.50％vs.88.89％),and the 3-year progression free survival rate of the low expression group of TFF1 was lower than that of the high expression group(57.14％vs.90.70％)(Log rank x2/P=13.551/＜0.001,15.310/＜0.001);Pathologically undifferentiated type and high serum LncRNA NEAT1 were risk factors affecting the prognosis of Rb,while high serum TFF1 was a protective factor[HR(95％)CI=1.523(1.147-2.024),1.473(1.108-1.957),0.612(0.413-0.908)];The area under the curve of serum LncRNA NEAT1,TFF1,and their combined prediction of Rb prognosis were 0.836,0.861,and 0921,respectively,which were larger than the single detection of serum LncRNA NEAT1 and TFF1(Z=4.823,4312,P＜0.001).Conclusion Serum LncRNA NEAT1 levels increase and serum TFF1 levels decrease in Rb patients,both of which are related to the maximum tumor diameter and pathological classification,and are factors affecting the prognosis of Rb patients.The combination of the two can help evaluate the prognosis of patients.
RetinoblastomaLong chain non coding RNA nuclear enriched transcript 1Trefoil factor 1Prediction of prognosisChildren
维普
万方数据
