Objective To explore type 3 β tubulin(TUBB3)and chaperone 8 containing TCP 1(CCT8)in colorectal cancer(CRC)and their correlation with epithelial mesenchymal transition(EMT)and prognostic significance.Methods From January 2018 to January 2020,112 patients with CRC were treated in the Department of Colorectal Surgery at the Affili-ated Traditional Chinese Medicine Hospital of Xinjiang Medical University were selected.Real time fluorescent quantitative PCR was used to detect TUBB3,CCT8 mRNA and EMT related indicators N cadherin(E cad),E cadherin(n cad),transcrip-tion factor twist mRNA expression.The expressions of TUBB3 and CCT8 proteins were detected by immunohistochemistry.The protein expressions of TUBB3 and CCT8 in CRC with different clinicopathological features were compared.Kaplan Meier curve and Cox regression analysis were used to analyze the effect of TUBB3 and CCT8 protein on the prognosis of CRC pa-tients.Results The expressions of TUBB3 mRNA,CCT8 mRNA,N cad mRNA and TWIST mRNA in CRC tissues were higher than those in adjacent tissues,while E cad mRNA was lower than that in adjacent tissues(t/P=35.030,38353,32.172,32.405,18.928,all P＜0.001).The positive rates of TUBB3 and CCT8 proteins in CRC tissues were 91.07％ (102/112),94.64％ (106/112),which were higher than those in adjacent tissues[7.14％ (8/112),625％ (7/112)],and the difference was statisti-cally significant(x2=157.836,175.032,P＜0.001).There was a positive correlation between tubb3 mRNA and cct8 mRNA ex-pression in CRC(r=0.647,P＜0.001).The expression of TUBB3 mRNA and CCT8 mRNA in CRC was positively correlated with the expression of N-cad mRNA and TWIST mRNA(r=0.667,0.621,0.703,0.686,P＜0.001),and negatively correlated with the expression of Ecad mRNA(r=-0.641,-0587,P＜0.001).The expressions ofTUBB3 mRNA and CCT8 mRNA in CRC with TNM stage Ⅲ,low differentiation and lymph node metastasis were significantly higher than those in CRC with stage Ⅰ～Ⅱ,high differentiation and no lymph node metastasis(t/P=20.327,20.455,21.101,15.121,14.985,15.759,all P＜0.001).The 3 year OS of CRC patients was 43 33％ (26/60)in the TUBB3 mRNA high expression group,which was lower than 70.97％ (44/62)in the low expression group(Log rank x2=8.792,P=0.003),while the 3 year OS of CCT8 mRNA high expression group was 42.86％ (27/63),which was lower than 72.88％ (43/59)in the low expression group(Log rank x2=10.970,P＜0.001)TUBB3 mRNA increased,CCT8 mRNA increased,TNM stage Ⅲ,poor differentiation and lymph node me-tastasis were independent risk factors for prognosis of CRC[HR(95％ CI)=1.334(1.103-1.613),1.322(1.108-1.577),1.435(1.161-1.773),1.368(1.115-1.677),1.315(1.054-1.641)].Conclusion TUBB3 and CCT8 are up-regulated in CRC,which can promote the malignant progression of CRC by promoting EMT,and are new markers for the prognosis of CRC.
维普
万方数据
