Objective To study the expression and clinical prognostic value of aortic carboxypeptidase like protein(ACLP)and collagen type Ⅺ alpha 1 chain(COL11A1)in pancreatic cancer.Methods From January 2019 to January 2021,88 patients with pancreatic cancer who underwent surgical treatment in Shaanxi Cancer Hospital and Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine were selected as the study subjects.Real time fluorescence quantitative PCR and immunohistochemistry were used to detect the expression of ACLP and COL11A1 mRNA and protein in cancer tis-sues and adjacent tissues.Pearson correlation analysis was used to analyze the correlation between ACLP mRNA and COL11A1 mRNA.Kaplan Meier method was used to analyze the effect of ACLP and COL11A1 expression on the survival and prognosis of patients with pancreatic cancer.Cox regression analysis was used to analyze the prognostic factors of pancreatic cancer.Results The relative expression levels of ACLP and COL11A1 mRNA in pancreatic cancer tissues were higher than those in adjacent tissues(t/P=31.058/＜0.001,27.642/＜0.001);The positive rates of ACLP and COL11A1 in pancreatic cancer tissues were 69.32％ (61/88)and 70.45％ (62/88),respectively,which were higher than 9.09％ (8/88)and 6.82％ (6/88)in adjacent tissues(x2=66.963,75.155,P＜0.001);The expression of ACLP mRNA was positively correlated with that of COL11A1 mRNA in pancreatic cancer tissues(r=0.642,P＜0.001);The positive rates of ACLP and COL11A1 protein in pancreatic cancer tissues with TNM stage ⅡB～Ⅲ and lymph node metastasis were higher than those in pan-creatic cancer tissues with TNM stage Ⅰ～ⅡA and no lymph node metastasis(ACLP:x2/P=19.704/＜0.001,12.908/＜0.001;COL11A1:x2/P=22.440/＜0.001,14.569/＜0.001).The 3-year overall survival rate of ACLP positive group was 22.95％ (14/61),which was lower than that of negative group 44.44％ (12/27)(Log rank x2=5.433,P=0.020);The 3-year overall survival rate of COL11A1 positive group was 20.97％ (13/62),which was lower than that of negative group 50.00％ (13/26)(Log rank x2=7281,P=0.007).TNM stage ⅡB～Ⅲ,lymph node metastasis,ACLP positive and COL11A1 positive were inde-pendent risk factors affecting the prognosis of patients with pancreatic cancer[HR(95％ CI)=1.781(1.199-2.646),1.962(1.172-3 285),1.505(1.066-2.125),1.568(1.117-2.201)].Conclusion The mRNA and protein expressions of ACLP and COL11A1 are significantly up-regulated in pancreatic cancer tissues.They play a promoting role in the occurrence and pro-gression of pancreatic cancer,and are new clinical markers for evaluating the prognosis of pancreatic cancer patients.
Pancreatic cancerAortic carboxypeptidase like proteinCollagen type Ⅺ alpha 1 chainPrognosis
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