Objective To study the expression of 3-phosphoglycerate dehydrogenase(PHGDH)and apolipopro-tein L3(APOL3)in colorectal cancer(CRC),and to analyze their prognostic value.Method A total of 112 patients with CRC admitted to the Department of Gastroenterology,Daxing Teaching Hospital of Capital Medical University from March 2018 to February 2021 were retrospectively collected.The expressions of PHGDH and APOL3 mRNA and protein in cancer tissues and adjacent tissues were detected by real-time fluorescence quantitative PCR(qPCR)and immunohistochemistry.Kaplan-Meier method was used to analyze the effect of PHGDH and APOL3 mRNA expression on the survival prognosis of CRC patients.Cox regression analysis was used to analyze the prognostic factors of CRC.The receiver operating characteristic(ROC)curve was used to evaluate the prognostic value of PHGDH and APOL3 mRNA expression levels in CRC patients.Results The relative expression of PHGDH mRNA in cancer tissues of CRC patients was higher than that in adjacent tis-sues,and the relative expression of APOL3 mRNA was lower than that in adjacent tissues(t/P=52.982/＜0.001,35.679/＜0.001);The positive rates of PHGDH and APOL3 protein in cancer tissues of CRC patients were 78.10％(90/112)and 8.57％(10/112),respectively,compared with 7.62％(8/112)and 76.19％(88/112)in adjacent tissues,the difference was statistically significant(x2/P=123.722/＜0.001,110.367/＜0.001);The expression level of PHGDH mRNA in CRC tissues with TNM stageⅢ and lymph node metastasis was higher than that in TNM stage Ⅰ-Ⅱ and no lymph node metastasis,while the expression level of APOL3 mRNA was lower than that in TNM stage Ⅰ-Ⅱ and no lymph node metastasis(t/P=52268/＜0.001,51315/＜0.001;15.873/＜0.001,14.769/＜0.001).The 3-year overall survival rate of PHGDH mRNA high expression group was 50.00％(26/52),which was lower than that of low expression group 73 33％(44/60)(Log rank x2=4.169,P=0.041).The 3-year overall survival rate of APOL3 mRNA low expression group was 46.30％(25/54),which was lower than that of high expression group 77.59％(45/58)(Log rank x2=10.650,P=0.001).TNM stage Ⅲ,lymph node metastasis,and high PHGDH mRNA were independent risk factors affecting the prognosis of CRC patients[OR(95％CI)=1.465(1.172-1.832),1.501(1.159-1.944),1.384(1.088-1.761)],and high APOL3 mRNA was an independent protective factor[OR(95％CI)=0.775(0.617-0.924)].The area under the curve(AUC)of PHGDH,APOL3 mRNA expression levels and the combination of the two in evaluating the prog-nosis of CRC patients were 0.830,0.816 and 0.922,respectively.The AUC of the combination of the two was greater than that of PHGDH and APOL3 mRNA expression levels alone(Z=4.482,4.130,P＜0.001).Conclusion The expression of PHG-DH is up-regulated and the expression of APOL3 is down-regulated in CRC,which plays an important role in promoting cancer in CRC.The combined detection of PHGDH and APOL3 expression is helpful to evaluate the prognosis of CRC.
万方数据
维普
