Objective To detect the NOC2L and p53 gene mRNA expression in laryngeal cancer tumor tissues and study their clinical value.Methods A total of 103 patients with laryngeal cancer(laryngeal cancer group)and 57 patients with benign laryngeal diseases(control group)from January 2020 to January 2022 were selected as the research subjects.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of NOC2L and p53 in tis-sues.The differences in the expression of NOC2L and p53 in tumor tissues of laryngeal carcinoma with different clinical pathological characteristics were compared.The ROC curve was used to analyze the sensitivity and specificity of NOC2L and p53 expression in predicting the poor prognosis of laryngeal cancer patients;the multivariate Cox regression model was used to analyze the risk factors for poor prognosis of laryngeal cancer;the Kaplan Meier model was used to analyze the effect of NOC2L and p53 expression on the survival of laryngeal cancer.Results Compared with the control group,NOC2L expression was increased and p53 expression was decreased in the laryngeal cancer group(t/P=19.307/＜0.001,13.726/＜0.001).NOC2L expression was higher in laryngeal cancer patients with histological grade 3,primary tumor diameter ≥3 cm,number of metastatic lymph nodes N2～N3,distant metastasis Ml and TNM stage Ⅲ～Ⅳ than in patients with histological grade 1～2,primary tumor diameter＜3 cm,number of metastatic lymph nodes N0～Nl,distant metastasis M0 and TNM stage Ⅰ～Ⅱ(t/P=3.681/＜0.001,4.167/＜0.001,2.554/0.012,4.005/＜0.001,3.228/＜0.001),while p53 expression was lower in patients with histological grade 1～2,primary tumor diameter＜3 cm,number of metastatic lymph nodes N0～N1,distant metastasis M0 and TNM stage Ⅰ～Ⅱ,number of metastatic lymph nodes N0～N1,distant metastasis M0 and TNM stage Ⅰ～Ⅱ patients(t/P=4.162/＜0.001,4.789/＜0.001,4.053/＜0.001,3.492/＜0.001,3.724/＜0.001);the AUCs ofNOC2L,p53 and their combination for predicting poor prognosis of laryngeal cancer were 0.756,0.712 and 0.917,respectively,and the combination of the two was superior to the single prediction efficiency(Z=7238,8.102,P＜0.001).NOC2L≥0.73,p53≤0.65,histological grade 3,primary tumor diameter ≥3 cm,number of metastatic lymph nodes N2～N3,distant metastasis Ml and TNM stageⅢ～Ⅳ were independent risk factors for poor prognosis of laryngeal cancer[HR(95％CI)=5.328(1.455-9.201),4.200(1279-7.122),1.992(1.127-2.857),2.164(1.099-3299),2228(1.304-3.152),2.406(1.131-3.681),2.514(1278-3.762)];The median survival of laryngeal cancer patients with NOC2L≥0.81 and p53≤0.54 was significantly lower than that of patients with NOC2L＜0.81 or p53＞054(median survival24.61±4.39 months vs.30.57±5.08 months,Log Rank=9.033,P＜0.001).Conclusion The expression of NOC2L and p53 in patients with laryngeal cancer is significantly related to the severity of the disease and survival time,which can provide objective evidence for the evaluation of the condition and prognosis of la-ryngeal cancer.The combination of NOC2L and p53 can significantly improve its clinical value.
Laryngeal cancerNOC2Lp53Tumor tissueClinical value
维普
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