Objective To investigate the relationship between the expression of long non-coding ribonucleic acid GATA binding protein 3 antisense RNA1(LncRNA GATA3-AS1)and micromas 362-3p(miR-362-3p)and pathological pa-rameters and prognosis in endometrial carcinoma.Methods One hundred and one patients with endometrial cancer admit-ted to Department of Pathology and the Department of Obstetrics and Gynecology of Chaohu Hospital Affiliated to Anhui Medical University,Department of Pathology of the Eighth People's Hospital of Hefei from January 2018 to December 2021 were selected as the study objects.Real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the mRNA expression levels of LncRNA GATA3-AS1 and miR-362-3p.The correlation between LncRNA GATA3-AS1 and miR-362-3p in endometrial cancer tissues was analyzed by Pearson correlation.Kaplan-Meier method was used to analyze the effects of LncRNA GATA3-AS1 and miR-362-3p expression on survival and prognosis of patients with endometrial cancer.Multivariate Cox regression analysis of the prognostic factors in patients with endometrial cancer.Results The ex-pression of LncRNA GATA3-AS1 and miR-362-3p in cancer tissues was higher than that in para-cancer tissues(t/P=17.642/＜0.001,18.153/＜0.001).The expression of LncRNA GATA3-AS1 in endometrial carcinoma was negatively correlated with the expression of miR-362-3p(r=-0.691,P＜0.001).LncRNA GATA3-AS1 expression in endometrial cancer tissues with FIGO stage ⅢA,myographic invasion depth＞1/2 and lymph node metastasis was higher than that in FIGO stageⅠ～Ⅱ myographic invasion depth ≤1/2 and no lymph node metastasis.The expression of miR-362-3p was lower than that in FIGO stages Ⅰ to Ⅱ,the depth of myographic infiltration was ≤ 1/2,and no lymph node metastasis was found(t/P=16.168/＜0.001,9.423/＜0.001,20.066/＜0.001,12.563/＜0.001,8.139/＜0.001,5.923/＜0.001).The 3-year overall survival(OS)rate in the high expression group of LncRNA GATA3-AS1 was 63.46％(33/52),which was lower than that in the low ex-pression group of 80.85％(38/47)(Log-rank x2=4.431,P=0.032).The 3-year OS rate of miR-362-3p group was 62.00％(31/50),which was lower than that of Mir-362-3p group(81.63％(40/49)(Log-rank x2=5240,P=0.022).FIGO stage ⅢA,depth of myographic invasion＞1/2,lymph node metastasis,and high LncRNA GATA3-AS1 were independent risk factors for prognosis of patients with endometrial cancer[HR(95％CI)=1.637(1.135-2.362),1.667(1.085-2.561),1.881(1.169-3.029),1.675(1.164-2.412)],miR-362-3p was an independent protective factor[HR(95％CI)=0.649(0.495-0.851)].Conclusion The expression of LncRNA GATA3-AS1 is up-regulated and the expression of miR-362-3p is down-regulated in endometrial carcinoma tissues,which is related to malignant pathological parameters and poor prognosis.LncRNA GATA3-AS1 may participate in the progression of endometrial cancer by negatively regulating miR-362-3p.
Endometrial cancerGATA binding protein 3 antisense RNA1MiR-362-3pPathological parametersPrognosis
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