Objective To investigate the expression level and clinical significance of serum sclerostin and n-3 fatty acid in elderly patients with sarcopenia.Methods One hundred and eighteen elderly patients with sarcopenia admitted to the Department of Geriatrics,the Fifth Affiliated Hospital of Xinjiang Medical University from May 2021 to May 2023 were collected as the case group,according to the severity of the disease,the patients were divided into pre-sarcopenia(n=39),sarcopenia(n=46)and severe sarcopenia(n=33),and 60 healthy subjects were selected as the control group.Serum sclerostin was detected by enzyme-linked immunosorbent assay,and n-3 fatty acid level was detected by gas chromatogra-phy.Body composition analyzer and bioelectrical resistance antibody method were used to detect body fat percentage,up-per arm circumference,visceral fat area and protein mass,the walking speed was measured by 6 m walking method,the grip strength of both hands was measured by Jamar grip strength meter,and the skeletal muscle mass of the whole body and limbs was measured by dual energy X-ray absorptiometry,and the relative skeletal muscle mass index(RSMI)of the limbs was calculated;the correlation of sclerostin and n-3 fatty acids with disease severity and body fat percentage,upper arm circumference,visceral fat area,protein mass,walking speed,grip strength,skeletal muscle mass and RASM was analyzed by Pearson product moment correlation or Spearman rank correlation;Logistic regression analysis was used to analyze the influencing factors of sarcopenia in the elderly.The diagnostic value of serum sclerostin and n-3 fatty acids for sarcopenia in the elderly was evaluated by ROC curve.Results Serum sclerostin and n-3 fatty acid levels in case group were less than those in control group(t/P=13.342/＜0.001,13.116/＜0.001).Serum sclerostin and n-3 fatty acid levels in pre-sarcopenia stage were higher than those in sarcopenia stage and severe sarcopenia stage(F/P=59.138/＜0.001,79.217/＜0.001).Per-centage of body fat,and visceral fat area in case group were greater than those in control group(t/P=8.732/＜0.001,5.124/＜0.001),upper arm circumference,protein mass,walking speed,grip strength,skeletal muscle mass of the whole body and limbs,RSMI in case group were less than those in control group(t/P=3.859/＜0.001,8.459/＜0.001,5.758/＜0.001,12.492/＜0.001,7.006/＜0.001,10.334/＜0.001,11.813/＜0.001).Serum sclerostin and n-3 fatty acid levels were negative associ-ated with percentage of body fat,and visceral fat area(r/P=-0.537/＜0.001,-0.612/＜0.001;-0.498/＜0.001,-0.523/＜0.001),and positive associated with upper arm circumference,protein mass,walking speed,grip strength,skeletal muscle mass of the whole body and limbs,RSMI(r/P=0593/＜0.001,0.624/＜0.001,0.639/＜0.001,0597/＜0.001,0.601/＜0.001,0.607/＜0.001,0.638/＜0.001;0569/＜0.001,0.611/＜0.001,0570/＜0.001,0592/＜0.001,0549/＜0.001,0534/＜0.001,0587/＜0.001).Age,body fat percentage,and increased visceral fat area are risk factors for sarcopenia in the elderly[OR(95％CI)=1.702(1.115-2.600),1551(1.052-2.287),1.387(1.006-1.913)],while BMI,upper arm circumference,protein mass,pace,grip strength,whole-body skeletal muscle mass,limb skeletal muscle mass,RSMI,osteocalcin,and n-3 fatty acids are protective factors for sar-copenia in the elderly[OR(95％CI)=0.728(0.539-0.982)、0.768(0.593-0.995)、0.845(0.723-0.986)、0.815(0.668-0.995)、0.585(0.382-0.897)、0.746(0.573-0.972)、0.733(0.559-0.964)、0.713(0.541-0.940)、0.822(0.695-0.973)、0.803(0.664-0.971)];The AUC of serum osteocalcin,n-3 fatty acids,and their combined diagnosis of sarcopenia in the elderly were 0.822,0.818,and 0.894,respectively.The AUC of their combined diagnosis of sarcopenia in the elderly was greater than that of their individual diagnosis(Z=2205,2.328,P=0.002,0.001).Conclusion Serum sclerostin and n-3 fatty acids decrease in elderly sarcopenia patients,which are related to disease progression.Early testing of two indicators can be used as bio-chemical indicators in clinical diagnosis of elderly sarcopenia patients.
维普
万方数据
