Objective To study the protective effect and mechanism of ginsenoside Rb1 on vascular endothelium of sepsis by regulating mitophagy through phosphatidylinositol-3 kinase/protein kinase B/glycogen synthase kinase 3β(PI3K/AKT/GSK3β)signaling pathway.Methods Healthy male Kunming mice were randomly divided into 5 groups:Sham operation group(Sham group),sepsis group(CLP group),ginsenoside Rb1+sepsis group(ginsenoside Rbl group),PI3K inhibitor LY294002+ginsenoside Rb1+sepsis group(LY294002 group)and mitophagy inhibitor Mdivi-1+ginsenoside Rb1+sepsis group(Mdivi-1 group).The sepsis mouse model was replicated by cecal ligation and puncture(CLP).Each group was treated with corresponding drugs.The general condition of mice was observed after operation.After 24 hours,aortic tissue samples were taken to observed the pathological changes by Hematoxylin-eosin(HE)staining and detected the expression levels of vWF,p-AKT,p-GSK3β and Parkin by immunohistochemistry.Results The general condition and ab-dominal infection of mice in the Sham group and the ginsenoside Rb1 group were mild,the intima structure of the aortic tis-sue was complete,the cells were arranged neatly,and the shape distribution was regular.In the CLP group and the inhibitor group,there were different degrees of intimal damage hyperplasia and disordered arrangement of the media,and different thickness of the wall.Immunohistochemical results showed that compared with the Sham group,the expression of vWF and Parkin in the CLP group was increased(P＜0.05),and the expression of p-AKT and p-GSK3β was decreased(P＜0.05).Compared with the CLP group,the expression of vWF in the ginsenoside Rb1 group was significantly decreased(P＜0.05),and the expression of p-AKT,p-GSK3β and Parkin was increased(P＜0.05).Compared with ginsenoside Rb1 group,the ex-pression of p-AKT,p-GSK3β and Parkin were decreased in LY294002 group(P＜0.05),and the expressions of p-AKT and p-GSK3β were not significantly different in Mdivi-1 group,but the expression of Parkin was significantly decreased(P＜0.05).Compared with LY294002 group,the expression of p-AKT and p-GSK3β was significantly increased(P＜0.05),and the expres-sion of Parkin was decreased in Mdivi-1 group(P＜0.05).Conclusion Ginsenoside Rb1 promotes mitophagy by activating PI3K/AKT/GSK3β signaling pathway,which has a protective effect on vascular endothelium in patients with sepsis.
维普
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