Objective To investigate the correlation between the expression of miR-122-5p and NOC2L in peripher-al blood and clinicopathological characteristics and prognosis of pancreatic cancer.Methods Patients with pancreatic cancer(PAC group,97 cases)and patients with benign pancreatic diseases(control group,53 cases)from January 2020 to January 2023 were selected as the research subjects.The expression of miR-122-5p and NOC2L in peripheral blood was de-tected by real-time fluorescence quantitative polymerase chain reaction.The differences in the expression of miR-122-5p and NOC2L in different clinicopathological characteristics were compared.The correlation between miR-122-5p and NOC2L and clinicopathological characteristics was analyzed by Spearman rank correlation.The sensitivity and specificity of miR-122-5p and NOC2L in predicting poor prognosis of pancreatic cancer were compared by ROC curve and DeLong method.Multivari-ate Cox regression was used to analyze the risk factors for poor prognosis of pancreatic cancer.Kaplan-Meier survival mod-el analysis and Log Rank comparison were used to compare the differences in survival among patients with different periph-eral blood miR-122-5p and NOC2L expressions.Results The expression of peripheral blood miR-122-5p in the PAC group was lower than that in the control group,while the expression of NOC2L was higher than that in the control group(t/P=28.061/＜0.001,29.701/＜0.001).The expression of miR-122-5p in peripheral blood of patients with histological grade 3,prima-ry tumor size ≥4 cm,number of metastatic lymph nodes N2,distant metastasis M1 and TNM stage Ⅲ-Ⅳ was lower than that of patients with histological grade 1-2,primary tumor size＜4 cm,number of metastatic lymph nodes N0-1,distant me-tastasis M0 and TNM stage Ⅰ-Ⅱ(t/P=5.935/＜0.001,2.801/0.006,3284/0.001,3.583/＜0.001,6.567/＜0.001),while the expres-sion of NOC2L was higher(t/P=2.039/0.044,3318/0.001,2287/0.024,2.417/0.018,3.943/＜0.001).The peripheral blood miR-122-5p expression in pancreatic cancer patients was negatively correlated with histological grade,primary tumor size,number of meta-static lymph nodes,distant metastasis and TNM stage(rs/P=-0.713/0.016,-0.678/0.021,-0.764/0.009,-0.695/0.011,-0.732/0.004),NOC2L expression was positively correlated with histological grade,primary tumor size,number of metastatic lymph nodes,distant metastasis and TNM stage(rs/P=0.657/0.039,0.701/0.014,0.726/0.019,0.672/0.028,0.717/0.015).miR-122-5p,NOC2L and their combination had an AUC of 0.735,0.719 and 0.863 for predicting poor prognosis of pancreatic cancer,re-spectively.The combination of the two was superior to their individual predictive efficacy(Z/P=9.412/＜0.05,10.013/＜0.05).miR-122-5p ≤0.69,NOC2L≥121,histological grade 3,primary tumor size ≤4 cm,number of metastatic lymph nodes N2,distant metastasis M1,and TNM stage Ⅲ-Ⅳ were independent risk factors for poor prognosis of pancreatic cancer[HR(95％CI)=4.051(1217-6.885),4.063(1.159-6.968),1.723(1.013-2.433),1.831(1.022-2.641),2.036(1.141-2.932),2.300(1.097-3.503),2.474(1.115-3.834)].The median survival of pancreatic cancer patients with miR-122-5p ≤0.69 and NOC2L≥ 1.21 was(10.39±2.45)months,which was significantly lower than that of other patients(miR-122-5p＞0.69 or NOC2L＜1.21)(15.61±3.08)months(Log Rank=12.573,P＜0.001).Conclusion The expression of miR-122-5p and NOC2L in peripheral blood of pan-creatic cancer is closely related to clinical pathological characteristics and prognosis,and has certain clinical value in prog-nosis and disease prognosis.The combined detection of the two can improve the sensitivity and specificity in predicting poor prognosis of pancreatic cancer.
维普
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