Objective To study the expression of nucleolin 2(NUCB2)and integrinβ3(ITGB3)in nasopharyngeal carcinoma(NPC)and their prognostic value in NPC patients.Methods The cancer tissues and adjacent tissues of 94 pa-tients with NPC who were admitted to the Department of Otolaryngology in the First Afiiliated Hospital of Naval Medical U-niversity from March 2017 to March 2018 were collected.Real-time fluorescence quantitative PCR(qPCR)and immunohisto-chemistry were used to detect the expression of NUCB2 and ITGB3 mRNA and protein in cancer tissues and adjacent tis-sues.Kaplan-Meier method was used to analyze the effect of NUCB2 and ITGB3 mRNA expression on the survival and prognosis of NPC patients.Cox regression analysis was used to analyze the prognostic factors of NPC patients.Results The positive rates of NUCB2 and ITGB3 protein expression in cancer tissues of NPC patients were 95.74％(90/94)and 8723％(82/94),respectively,which were higher than 8.51％(8/94)and 10.64％(10/94)in adjacent tissues(x2/P=143 323/＜0.001,29.139/＜0.001);The relative expression of NUCB2 and ITGB3 mRNA in cancer tissues of NPC patients was higher than that in adjacent tissues,and the difference was statistically significant(t/P=49.760/＜0.001,43.704/＜0.001);The ex-pression levels of NUCB2 and ITGB3 mRNA in NPC tissues of TNM stage Ⅲ～Ⅳ were higher than those of TNM stageⅠ～Ⅱ(t/P=17.443/＜0.001,14275/＜0.001).The 5-year overall survival rate of NUCB2 high expression group was 52.17％(24/46),which was lower than that of NUCB2 low expression group(83.33％,40/48)(Log-rank x2=10.941,P=0.001);The 5-year overall survival rate of ITGB3 high expression group was 53.33％(24/45),which was lower than 81.63％(40/49)of ITGB3 low expression group(Log-rank x2=9.832,P=0.002).TNM stage Ⅲ～Ⅳ,increased NUCB2 mRNA and increased ITGB3 mRNA were independent risk factors affecting the prognosis of NPC patients[OR(95％CI)=1.608(1225-2.112),1.839(1228-2.753),1.738(1246-2.426)].Conclusion The mRNA and protein expressions of NUCB2 and ITGB3 are up-regulated in NPC tissues.They are involved in the progression of NPC tumors and are markers for evaluating the prognosis of NPC patients.
维普
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