Objective To investigate the correlation between the expression of chaperonin containing TCP1 sub-unit 5(CCT5)and metalloproteinase associated protein 1(OMA1)in gastric cancer(GC)tissue and epithelial mesenchymal transition(EMT),and to analyze their clinical prognostic value.Methods From February 2019 to February 2020,104 patients with GC diagnosed and treated in the Department of Gastroenterology of Wuhan Third Hospital were selected.The expres-sion of CCT5 mRNA,OMA1 mRNA and EMT markers Twist mRNA,N-cad mRNA and Vimentin mRNA were detected by real-time fluorescence quantitative PCR;The expression of CCT5 and OMA1 protein was detected by immunohistochemis-try;Pearson correlation analysis was used to analyze the correlation between CCT5 mRNA,OMA1 mRNA and EMT inde-xes;Kaplan-Meier curve and COX regression were used to analyze the effects of CCT5 mRNA and OMA1 mRNA on the prognosis of GC patients.Results CCT5 mRNA,OMA1 mRNA,Twist mRNA,N-cad mRNA and Vimentin mRNA in GC cancer tissues were higher than those in adjacent tissues(t/P=71.623/＜0.001,81.587/＜0.001,38.717/＜0.001,52234/＜0.001,44.813/＜0.001).The positive rates of CCT5 and OMA1 in GC cancer tissues were 86.54％(90/104)and 88.46％(92/104),which were higher than those in adjacent tissues 5.77％(6/104)and 7.69％(8/104)(x2=136.500,135.893,P＜0.001).The ex-pression of CCT5 mRNA and OMA1 mRNA in GC was positively correlated with Twist mRNA,N-cad mRNA and Vimentin mRNA(r/P=0.754/＜0.001,0.612/＜0.001,0.649/＜0.001;0.716/＜0.001,0.695/＜0.001,0.713/＜0.001);The expression levels of CCT5 mRNA and OMA1 mRNA were increased in GC patients with worse differentiation,lymph node metastasis and higher TNM stage(CCT5:t/F/P=302.221/＜0.001,12.763/＜0.001,244.850/＜0.001;OMA1:t/F/P=402.771/＜0.001,13.069/＜0.001,305.421/＜0.001);The 3-year overall survival rate of CCT5 mRNA high expression group was 44.00％(22/50),which was lower than 75.93％(41/54)of low expression group,the difference was statistically significant(Log-Rank x2=7.156,P=0.008).The 3-year overall survival rate of OMA1 mRNA high expression group was 3922％(20/51),which was lower than 81.13％(43/53)of low expression group(Log-Rank x2=8.901,P=0.003).Poor differentiation,lymph node metastasis,TNM stage Ⅲ,high CCT5 mRNA and high OMA1 mRNA were risk factors affecting the prognosis of GC patients[OR(95％CI)=1.554(1217-1.986),1.670(1274-2.189),1.751(1.302-2.354),1.495(1.105-2.021),1.483(1.079-2.037)].Conclusion The ex-pression of CCT5 and OMA1 in GC is up-regulated,which is related to EMT indicators and is a tumor marker for evaluating the prognosis of GC patients.
维普
万方数据
