Taking the soft cuttings from saplings of Nyssa yunnanensis as the materials,the influences of media,concentrations of ABT1 and position from which cuttings were collected on rooting were studied.The results showed that sand was the most promising media,the highest cutting ratio and root number were 64 % and 8 respectively.Using the ABT1 of 500 mg/L,the rooting ratio was 7.5 % higher than the control.The cuttings with tip shoot was the best type,significant differences were existing with other types of cuttings on rooting ratio,average root length and root number.It was found that the rooting of Nyssa yunnanensis occurred at cambium region.