首页|HPLC分析6种不同花色滇牡丹花瓣中花青素和黄酮

HPLC分析6种不同花色滇牡丹花瓣中花青素和黄酮

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滇牡丹是牡丹野生种之一,具有非常丰富的颜色,有白色、黄色、黄绿色、橙色、红色(深红、紫红)、紫色和紫黑色等颜色.采用HPLC和HPLC-DAD-ESI-MS2同时分析6种不同花色滇牡丹花瓣中花青素和黄酮成分,分析并鉴别出4种花青素化合物和7种黄酮化合物.研究表明:除黄色花瓣,其余5种颜色的花瓣中都含有相同种类的花青素,不同颜色花瓣中花青素的相对百分含量差距比较大.首次从滇牡丹粉色系花瓣中分析并鉴定出矢车菊素双葡萄糖苷(cyanidin-3,5-di-O-glucoside)、矢车菊素单葡萄糖苷(cyanidin-3-O-glucoside)、芍药素双葡萄糖苷(peonidin-3,5-di-O-glucoside)和芍药素单葡萄糖苷(peonidin-3-O-glucoside).6种颜色滇牡丹花瓣中黄酮化合物的种类几乎相同,仅含量有差异.
Analysis of Anthocyanins and Flavonols in six Different Colors of Petals of Paeonia delavayi by High Performance Liquid Chromatography
Paeonia delavayi is one of the wild species of tree peony,which has rich colors including white,yellow,yellow-green,orange,red (dark-red,purple-red),purple and purple-black.In our study,the anthocyanin and flavonol from 6 kinds of Petals of Paeonia delavayi were analyzed by using high-performance liquid chromatography (HPLC) and high-performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-ESI-MS2).4 anthocyanin compounds and 7 flavonol compounds were tentatively identified.The resuits indicated that excepting the yellow petals,the other 5 kinds of petals have the same anthocyanins and the relative percentage of anthocyanins have significant difference in different colors of petals.Cyanidin-3,5-di-O-glucoside,cyanidin-3-O-glucoside,peonidin-3,5-di-O-glucoside and peonidin-3-O-glucoside were first analyzed in pink Paeonia delavayi.There were no obvious differences in the composition of flavonol among the 6 kinds of petals.

Paeonia delavayianthocyaninsflavonolHPLC-DAD-ESI-MS2

华梅、原晓龙、杨卫、陈剑、呼延丽、谭芮、杨宇明、王娟

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云南省林业科学院,云南昆明650201

国家林业局云南珍稀濒特森林植物保护和繁育实验室/云南省森林植物培育与开发利用重点实验室,云南昆明650201

滇牡丹 花青素 黄酮 HPLC-DAD-ESI-MS2

云南省应用基础研究计划青年项目云南省科技计划(对外科技合作计划—国际科技合作)云南省森林植物培养与开发利用重点实验室开放基金

2014FD0692015IA005

2017

西部林业科学
云南省林业科学院 云南省林学会

西部林业科学

CSTPCD北大核心
影响因子:0.807
ISSN:1672-8246
年,卷(期):2017.46(6)
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