Effect of Insect-resistance Transgenic 107 Poplar on Rhizosphere and Endogenous Bacterial Communities
In order to research transgenic poplar 107 effect on in rhizosphere soil and control soil,we used high-throughput sequencing technology to compare and analyze the structure and diversity of soil bacteria,phloem and root endophytic fine communities of transgenic poplar 107 transgenic in rhizosphere soil and control soil.Sequencing results showed that the effective sequences in soil samples averaged more than 30 000,and those in poplar tissues averaged more than 300 000.The species composition of transgenic strains is extremely similar to that of the control strain.In the rhizosphere soil samples,Acidobacteria and Proteobacteria was the dominant phyla of transgenic and non-transgenic strains,accounting for 16.6％and 21.9％,respectively.In phloem samples,Cyanobacteria and Pro-teobacteria were the dominant phyla of transgenic and non-transgenic strains,accounting for 54.8％and 59.2％,re-spectively.In the root samples,Proteobacteria were the dominant phyla of transgenic and non-transgenic strains,ac-counting for 40.7％and 58.8％,respectively.There was no significant difference in the richness and diversity of bacterial community between transgenic and non-transgenic strains,but there was significant difference between dif-ferent parts.Through diversity analysis,it was found that the communities of transgenic and control strains had high similarity,while the communities of samples from different parts had certain differences.The results of this study showed that transgenic poplar 107 had no significant effects on rhizosphere soil and endophytic bacteria,and clearly presented the characteristics of bacterial communities in different parts of transgenic poplar 107,which provided a reference for biosafety evaluation of transgenic poplar 107.A reliable and practical method has been proposed to ef-fectively address insect resistance in the cultivation of new transgenic poplar varieties.
万方数据
维普
