Unexpected Effects of Transgenic BtCry1Ac on Timber Properties of Populus× euramericana'74/76'
The purpose of this study was to explore the unexpected effects of exogenous BtCry1Ac gene on wood properties of Populus × euramericana'74/76'(poplar 107).The wild type(WT)and two overexpression BtCrylAc lines(ECA1 and ECA2)of poplar 107 were selected,and their stems were used as materials.The chemical compo-sition,physical properties,mechanical properties,and fiber morphology of the stems were quantitatively meas-ured.The results demonstrated that heterologous expression of BtCry1Ac caused an unexpected variation of the tim-ber properties(i.e.,enhanced the timber's mechanical strength and drying shrinkage)in poplar 107.In details,the contents of the cellulose,ash,benzene alcohol extract,and 1％(v/v)NaOH extract of the ECA1 and ECA2 lines were lower(P＜0.05)than those of the WT line.The indexes of air/total dry shrinkage of the two transgenic lines were lower(P＜0.05);and the air/total dry densities were higher than those of the WT line.Furthermore,the impact toughness,flexural strength,flexural elastic modulus,and cis-grain compressive strength in the two OE-BtCry1Ac lines were both higher(P＜0.05)compared to the WT line.Moreover,the fiber lengths of the two OE-BtCry1Ac lines were both higher than those of the WT line(P＜0.05).As a whole,the wood of transgenic lines has less drying shrinkage,higher density and strength,and its wood properties have changed unexpectedly.The re-sults provide a reference for the popularization and application of transgenic poplar.
万方数据
维普
