首页|9种狼尾草属植物叶绿体基因组特征分析

9种狼尾草属植物叶绿体基因组特征分析

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狼尾草属为禾本科1 a生或多年生草本植物,该属植物在饲用、保护生态、观赏等方面均有良好的应用价值.为探讨狼尾草属植物叶绿体基因组的遗传结构及进化特征,基于基因组浅层测序技术获得狼尾草属9种植物叶绿体基因组序列,对其组装与注释后进行生物信息学分析.结果显示:(1)9种狼尾草属植物叶绿体基因组均为典型的四分体结构,其大小在137 929~138 554 bp之间.叶绿体基因组GC含量范围为38.6%~38.7%,其中IR区GC含量最高,为44%~44.1%,各叶绿体基因组结构无明显差异,在IR/SC交界区表现出微小差异.(2)共注释113个基因,包括78个编码蛋白基因、31个tRNA基因和4个rRNA基因.对序列进行简单重复序列检测,共检测到389个SSRs位点,其中单核苷酸重复数量最多,而在非简单序列重复检测中,串联重复所占比例最高.(3)变异位点主要存在于叶绿体LSC和SSC区,筛选出9个差异较大的区域,可作为狼尾草属植物的潜在遗传标记.(4)基于31条狼尾草属植物样本叶绿体基因组序列构建了狼尾草属系统发育树,结果形成两大分支,阐明了狼尾草属植物种间亲缘关系.本研究可为狼尾草属植物的分类鉴定、系统进化、种群遗传和分子育种等提供理论指导.
Chloroplast Genome Characteristics of Nine Pennisetum Plants Species
Pennisetum is an annual or perennial herb of Poaceae,and it has good application in feeding,ecological protection and ornamental.To investigate the genetic structure and evolutionary characteristics of the chloroplast ge-nome,the chloroplast genome sequences of 9 Pennisetum plants were obtained,and bioinformatics analysis was car-ried out.The results showed that the chloroplast genomes of Pennisetum nine plants were typical tetrad structures,ranging from 137 929 bp to 138 554 bp.The GC content of the chloroplast genome ranged from 38.6%to 38.7%,with the highest GC content in the IR region(44%-44.1%).There was no significant difference in the chloroplast genome structure,but there was a small difference in the IR/SC junction region.In addition,113 genes were annota-ted,including 78 protein-coding genes,31 tRNA genes and 4 rRNA genes.A total of 389 SSRs were detected by simple sequence repeat detection,among which the number of single nucleotide repeats was the largest,while the proportion of tandem repeats was the highest in non-simple sequence repeat detection.In Pennisetum species,the variation sites mainly existed in chloroplast LSC and SSC regions,and 5 highly divergent regions were successfully i-dentified that could be used as potential genetic markers of Pennisetum.Based on the phylogenetic tree constructed by chloroplast genome,Pennisetum plants formed two major branches,which clarified the genetic relationship be-tween some species of Pennisetum plants.This study can provide theoretical guiding for the classification and identi-fication,systematic evolution,population genetics and molecular breeding of Pennisetum species.

Pennisetumchloroplast genomesequence analysisphylogeny

胥富强、王海洋、师春娟、辛培尧、段利武、王齐

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甘肃省小陇山林业调查规划院,甘肃 天水 741020

西南林业大学林学院,云南 昆明 650224

云南林业职业技术学院,云南 昆明 650000

狼尾草属 叶绿体基因组 序列分析 系统发育

云南省教育厅科研项目

2022J1382

2024

西部林业科学
云南省林业科学院 云南省林学会

西部林业科学

CSTPCD北大核心
影响因子:0.807
ISSN:1672-8246
年,卷(期):2024.53(1)
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