Effects of adding Cinnamomum kaneirae and Cinnamomum burmanni Matrix on the Biosynthetic Pathway of Terpenoid Compounds of Taiwanofungus gaoligongensis
Taiwanofungus camphoratus is a rare edible and medicinal fungus.Its main components terpenoids have anticancer effects.In order to study the effects of adding Cinnamomum kaneirae and C.burmanni leaves matrix on the terpenoids of T.gaoligongensis,this study used Illumina high-flux sequencing platform to perform transcriptome sequencing on the mycelium of C.kaneirae,C.burmanni leaves and Coicis Semen(control group)matrix cultured in liquid medium,and the expression of key genes in the pathway of terpenoid synthesis were analyzed.The results showed that there were 446 differentially expressed genes,261 up-regulated and 185 down-regulated,when BSNZZ-NF and BSNZZ-YF were added.The GO enrichment analysis of DEGs showed that the genes related to oxidation-reduction process and various oxidoreductase activity were significantly enriched.KEGG pathway enrichment analy-sis showed that DEGs was significantly enriched in three metabolic pathways:Tyrosine metabolism,Propanoate me-tabolism,Glyoxylate and dicarboxylate metabolism.In the transcriptome data of T.gaoligongensis,a total of 43 meva-lonate pathway(MVA pathway)involved in the synthesis of terpenoids were screened.After adding C.kaneirae,the expressions of HBD9,HMG-CoA,GGT3 and OCS were significantly up-regulated,while the expressions of HBD12,TPS5 and SE genes were down-regulated.The expression levels of AACT1,HBD5,HBD8,HBD12 and FPPS1 were significantly up-regulated,while the expression levels of HMGS,HMG-CoA and IDI were down-regulated after the addition of C.burmanni.There were 14 transcription factors,including bHLH,bZIP and MYB,which were related to the biosynthesis of terpenoid compounds of T.gaoligongensis.The 14 transcription factors were expressed in different degrees after adding C.kaneirae and C.burmanni matrix.This study provides a reference for further elucidating the mechanism of transcription factors involved in the terpenoid biosynthesis of T.gaoligongensis.
Taiwanofungus gaoligongensistranscriptome analysisbiosynthesis of terpenoidstranscription factor
万方数据
维普
