Transcriptome Analysis on Formation of Adventitious Root during Olive Horizontal Press Seedling
Exploring the changes in gene expression regulation during the rooting process of olive(Olea europaea)could lay a foundation for screening key genes that control adventitious root development.Using the olive variety O.europaea'Tianyuan 1'as the experimental object,rooting powder 1(1500 mg/kg)was used to treat olive cut-tings.The rooting process was observed and transcriptome sequencing was performed on samples taken at 0,10,20,30 and 40 days after the cuttings were pressed.The expression of differentially expressed genes at different stages of olive rooting process was analyzed,and important metabolic pathways involved in olive rooting process were identi-fied.The results showed that:(1)There were 1218 genes(P<0.05)with significant differences in expression during the rooting process between YT0 and YT10,including 829 up-regulated and 389 down-regulated genes;Between YT10 and YT20,there are a total of 1157,including 567 up-regulated and 590 down-regulated;Between YT20 and YT30,there are a total of 1163,including 499 up-regulated and 664 down-regulated;There are a total of 1458 between YT30 and YT40,including 952 up-regulated and 506 down-regulated.(2)Through differential gene GO functional annotation analysis,it was found that significantly enriched terms include cell membrane,catalytic ac-tivity,oxidoreductase activity,cofactor binding,transport activity,transmembrane transport protein activity,carbohy-drate metabolism processes,etc.(3)By analyzing the KEGG enrichment analysis of differentially expressed genes,it was found that the main pathways involved in the rooting process include plant hormone signal transduction,phenyl-propanoid biosynthesis,starch and sucrose metabolism,conversion of pentose and glucuronic acid,amino and nucle-otide sugars,ribosomes,cysteine and methionine metabolism,amino and nucleotide sugars,etc.The results indicate that genes related to plant hormone signal transduction during the rooting process include ARF,GH3,AUX1,SAUR,JAZ,CYCD3,MYC2,etc.while genes related to phenylpropanoid biosynthesis pathway include β-Glucosidase trans cinnamate 4-monooxygenase,phenylalanine ammonia lyase,etc.Transcription factors participating in the rooting process encompass families such as bHLH,MYB,ERF,NAC,C2H2,and WRKY.
Olea europaeapressure stripformation of adventitious rootstranscriptomics
万方数据
维普
