Ultrasonic-assisted Ethanol Extraction of Flavones from Chimonocalamus fimbriatus Leaves and Their Removal and Blocking Effects on Nitrosation Reaction
Ultrasonic-assisted Ethanol Extraction of Flavones from Chimonocalamus fimbriatus Leaves and Their Removal and Blocking Effects on Nitrosation Reaction
In order to improve the yield of flavonoids from Chimonocalamus fimbriatus leaves,the response surface method was used to optimize the ultrasonic-assisted ethanol extraction of flavonoids from C.fimbriatus leaves.The antioxidant,nitrosation-removing and nitrosation-blocking abilities of the extracts were measured.The results showed that the optimal conditions of response surface optimization were ethanol volume fraction of 66.5%,solid-liquid ratio of 1︰30,ultrasonic power of 311 W,and the yield of flavonoids from bamboo leaves was 37.146 mg/g.The biological activity study showed that the scavenging ability of bamboo leaf extract on hydroxyl radical and su-peroxide anion was stronger than L-ascorbic acid(Vc),and the scavenging ability of DPPH free radical was slight-ly weaker in certain concentration range.The removal rate of nitrite can reach 52.59%,and the blocking rate of ni-trosation can reach 74.75%.The correlation analysis showed that flavonoids were the main antioxidants in C.fimbriatus leaves,and the removal of nitrosation and blocking of nitrosation reaction were highly correlated.This study found that the flavonoids of phyllostachys argifolia have great potential as multi-target anticancer drugs or health care products,and can promote the development of high value-added products of C.fimbriatus.
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