An Improved Method for Preparation Leaf Scraping with Varied Trichomes and Textures of Some Seed Plants
In order to improve a seed plant leaf epidermal section method suitable for observing leaf epidermal characteristics with different trichome and texture,6 representative seed plants with papery and leathery leaves were selected as the research materials.They were soaked in a mixture of dilute nitric acid and glacial acetic acid and stirred to remove the trichomes(or scales)on the leaf surface.Then,the upper and lower epidermis were separated in a mixture of glacial acetic acid and hydrogen peroxide,and transparent with lactic acid and stained with 1%saf-ranine.After sealing,the leaf epidermal features were observed under an optical microscope and compared with scanning electron microscopy characteristics.The results showed that:(1)digital cameras can be used to observe the surface of the leaves,and it can be seen that the surface of the leaves of 6 plant species in 2 textures is relatively smooth,without trichome or obvious trichomes;observing the lower surface of the leaves under a stereomicroscope,it can be seen that the Mentha canadensis leaves and Plantago asiatica leaves are relatively flat without glandular tri-chomes.The leaves of Artemisia lavandulifolia are densely covered with arachnoid tomentum,while the leaves of Elaeagnus pungens are densely covered with scales.The leaves of Crataegus pinnatifida can be seen with various levels of network veins,and the leaves of Ilex cornuta can be seen with"black spots"like sunken stomata.(2)Impro-ving the method of leaf epidermal sectioning can effectively remove the trichomes or scales on the surface of papery and leathery leaves,and separate the upper and lower epidermis.It can clearly observe leaf epidermal features that cannot be observed under scanning electron microscopy,including epidermal cell shape,vertical wall pattern,glan-dular trichome,and stomatal type.(3)For leathery leaves with thick cuticle and paper leaves or leather leaves with thick trichomes(or scales),scanning electron microscopy has high resolution and can clearly observe the texture of the cuticle or wax particles and trichomes or scales,but cannot observe the vertical wall pattern and stomatal type of epidermal cells;optical microscopy can observe the characteristics of epidermal cells such as the vertical wall pat-tern,stomatal type,and trichome type,but cannot observe the texture of cuticle or wax layer particles,as well as tri-chomes or scales.It can be concluded that the new method of leaf epidermal sectioning can effectively separate the upper and lower epidermis of papery leaves and leathery leaves,thereby obtaining ideal leaf epidermal section that can clearly observe the characteristics of leaf epidermis.
leaf epidermissection methodlight microscopescanning electron microscope
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