Bacterial Community Diversities in the Rhizosphere and Root of Pinus yunnanensis at Different Altitudes based on High-throughput Sequencing
The species,community structure and diversity of bacteria in rhizosphere soil and roots of Pinus yunnanen-sis at different altitudes were studied to provide some microbial evidence for the study of the adaptive mechanism of P.yunnanensis at different altitudes.The bacterial 16S rRNA gene v5-v7 region of P.yunnanensis roots and rhizo-sphere soils at different elevations was sequenced by high-throughput sequencing technology,and the community structure,diversity and function were analyzed.Based on high-throughput sequencing analysis,41 phyla,118 classes,277 orders,465 families,893 genera,1755 species of endophytic bacteria were identified from the roots of P.yunnanensis,and 34 phyla,96 classes,230 orders,395 families,761 genera were identified from the rhizosphere soil,1626 species.The Shannon Index of bacterial community in the rhizosphere soil of P.yunnanensis was signifi-cantly higher than that in the root.The most abundant bacteria in the rhizosphere soil were Proteobacteria and the most abundant Actinomycetes in the root,the relative abundance of Actinomycetes and Firmicutes in Roots was twice that in rhizosphere soil,and the relative abundance of Acidobacteria in rhizosphere soil was twice that in roots,in ad-dition,the metabolic functional abundance of endophytic bacteria community in root was higher than that in rhizo-sphere soil.The β-diversity of rhizosphere soil and root endophytic bacterial community of P.yunnanensis was signifi-cantly different at different altitudes(P<0.05),and the relative abundance of dominant species was different at differ-ent altitudes.The function prediction of PICRUST2 showed that the gene expression level of rhizosphere bacteria in-creased with the elevation gradient,and the gene expression level of root endophytic bacteria was only high at 2900 m.In conclusion,the diversity of endophytic bacteria in the rhizosphere soil and roots of P.yunnanensis was studied un-der different altitude gradients,this may be related to the adaptation of P.yunnanensis to the external environment.This study provides some data support for further exploring the environmental adaptation mechanism of P.yunnanensis.
万方数据
维普
