Effects of Nitrogen Deficiency on Physiology and Biochemistry in vitro Plantlets of 84K Poplar
The effect of nitrogen deficiency on in vitro plantlets of 84K poplar(Populus alba×P.glandulosa)was studied in order to provide a theoretical basis for the breeding and reproduction of 84K poplar.The biomass,photo-synthetic parameters and chlorophyll fluorescence parameters in vitro plantlets of 84K poplar under nitrogen defi-ciency were measured,and the changes of total nitrogen,total carbon,carbon-nitrogen ratio,total phenol and antiox-idant activity were further analyzed.The results showed that as the nitrogen content in the culture medium de-creased,the dry weight,net photosynthetic rate(Pn),and stomatal conductance(Gs)in vitro plantlets of 84K poplar were inhibited.At the same time,nitrogen deficiency reduced Chla,Chlb,Chl content and maximal photochemical efficiency(Fv/Fm)in vitro plantlets of 84K poplar.In addition,with the decrease of nitrogen in the medium,the to-tal nitrogen content decreased significantly,and the carbon-nitrogen ratio and the total phenol content increased sig-nificantly,in in vitro plantlets of 84K poplar.The results of antioxidant analysis showed that the lower the nitrogen content in the medium,the better the antioxidant activity of the in vitro plantlets.Correlation analysis showed that bi-omass was positively correlated with photosynthetic parameters and chlorophyll content,but negatively correlated with total phenol content.There was negative correlation between total nitrogen content and total phenol content,and positive correlation between total carbon content and carbon-nitrogen ratio.At the same time,there was a significant correlation between total phenol content and antioxidant activity of in vitro plantlets of 84K poplar.The results showed that nitrogen deficiency could affect the photosynthetic physiology and secondary metabolites of in vitro pla-ntlets of 84K poplar,which was not conducive to the growth and photosynthesis of in vitro plantlets of 84K poplar,but promoted the production of polyphenols and other antioxidants.This study can provide theoretical basis and bas-ic data for the cultivation and management of 84K poplar.
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