Objective To explore the application value of fungal antigen detection(G test and GM test)in invasive fungal infection.Methods A retrospective analysis was conducted on the test results of 2020 patients who underwent both G and GM tests,as well as fungal culture of related infection sites from Yuxi People's Hospital from January 2023 to August 2023.Based on the results of the G and GM tests,the patients were divided into three groups,and a chi square test was used to compare the data of the three groups.The impact of single detection and combined detection on improving fungal detection efficiency was analyzed.Results Among the 2020 patients,310 tested positive for fungal culture,including 290 cases of yeast and 20 cases of other fungi.151 cases were positive in the G test,of which 63 cases were also positive in the GM test.The analysis showed that when both G test and GM test were positive,the fungal detection rate was higher than when G test was positive and GM test was negative,and both were higher than when G test was negative.The fungal detection rate of G test positive group was significantly higher than that in the G test negative group.Conclusions The combined detection of fungal antigens can improve the detection rate of fungi,and has high clinical application value in the diagnosis of invasive fungal infections.It can be used as an auxiliary diagnostic indicator for invasive fungal infections.