Function of transient receptor potential cation channel TRPC1 in neural stem cells
Objective To elucidate the molecular mechanism on the specific TRPC1 expression in nerve cells,and provide a novel biological marker for depression research.Methods First,embryonic stem cell(ESCs)was induced to differentiate into neural stem cells in the medium.TRPC1 overexpression virus was packaged with retroviral vector by calcium transfer to infect neural stem cells.Then TRPC1 gene knock-out ESCs were constructed and induced to differentiate into TRPC1gene knock-out neural stem cells.Obtained normal neural stem cells,TRPC1 gene knock-out neural stem cells and TRPC1 over-expressive neural stem cells,three cell lines were treated with the addition 10ug/mL dexamethasone,and the proliferation number and 96h cell number were counted respectively.Results The proliferation ability of TRPC1 gene knock-out neural stem cell line was weakened under the normal culture medium;There was no significant effect on the proliferation of TRPC1 cells either after overexpression or knockout.Conclusions It can be concluded that TRPC1 may play a neuroprotective role by maintaining the proliferation ability of neural stem cells.
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