Study on the Purification and Extraction Technology of Bergenin from the Bergama purpurascens (Hook.f.et Thoms.) Engl
Objective : To optimize the best Extraction process and to investigate its Purification Technology of Bergenin from the Bergama purpurascens (Hook.f.et Thoms.) Engl.Methods: To determine Bergenin content with UV - visible spectrophotometer as an indicator to study the main effect factors of influencing macro reticular resin purification and ethanol extract Lechnology.Results: Having determined the optimal extraction process of Bergama purpurascens (Hook.f.et Thoms.) Engl., which include extraction Bergama purpurascens (Hook.f.et Thoms.) Engl.with tenfold 50％ ethanol three times, 0.5 hour per time, and best purification condition is that loading 2.5 grams net raw medicinal materials at most into D - 101 resin each gram and absorb 30mins and the procedure of elution is 6 times 20％ ethanol the volume of colume.Conclusion: the content of Bergenin reached 14.023％ , which based on The best parameters of purification and extraction, the results are reliable.
NETL
NSTL
万方数据
维普
