正交试验法优选黄芪中总黄酮提取工艺
Studing on Extraction Condition of Total Flavonoids from Radix Astragali by Orthogonal Test Method
赵琦 1张军武2
作者信息
- 1. 咸阳市卫生学校,陕西咸阳712000
- 2. 陕西中医学院,陕西咸阳712046
- 折叠
摘要
目的:优选黄芪总黄酮的提取工艺条件.方法:采用正交试验法对黄芪总黄酮提取工艺条件进行优选,以总提物和总黄酮含量为指标,考察影响提取指标的4个因素,即溶媒用量(A),乙醇浓度(B),提取时间(C),提取次数(D);每个因素取3个水平.结果:用乙醇提取黄芪总黄酮的最佳工艺为A2B2C1D2,即用10倍量70％乙醇回流提取2次,每次60min.结论:此法稳定可行,可为黄芪总黄酮提取工艺的确定提供依据.
Abstract
Objective; Select the best condition for extraction process of total flavonoids from Radix Astragali. Method; Regard the total extract yield and the yield of total flavonoids as indicators, total flavonoids extraction conditions were optimized by using orthogonal test method. Four factors were examined by three levels; the a-mount of solvent ( A), ethanol concentration ( B ) , extraction time ( C), extraction frequency (D). Results: The best extraction process of total flavonoids from Radix Astragali by ethanol is A2B2C1D2, that is, it was extracted with 10 times the amount of 70% ethanol two times, 60min every time. Conclusion: The method can provide a stable basis for extraction process of total flavonoids from Radix Astragali.
关键词
正交试验法/黄芪/总黄酮/提取工艺Key words
orthogonal test method/radix Astragali/total flavonoids/extraction process引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据