Objective Establishing a HPLC method for the determination of monoester aconitum alkaloids in paste of Fuqi Guben.Methods Column Waters XTerra RP18 5mm (4.6mm×250mm);column temperature was 30℃;Acetonitrile-tetrahydrofuran (25:15) as the mobile phase A,0.1mol/L ammonium acetate solution (1000mL containing glacial acetic acid per 0.5mL) as the mobile phase B,gradient elution,flow rate was 1 mL/min,the detection wavelength was 254 nm.Results Benzoylmesaconine is in the range of 0.045 ～1.800μg showed a linear relationship,benzoylaconitine is in the range of 0.0425～1.7000.μg showed a linear relationship,and benzoylhypacoitine is in the range of 0.0442 ～1.7680μg showed a linear relationship.Precision,stability,reproducibility are in line with the requirements,the average recovery of this measurement method were 99.89％,99.22％,101.12％,RSD was 1.72％,1.75％,2.32％(n=6).Conclusion The method was so convenient,fast,accurate and no interference that it can be used for quality control of the preparation.
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