The Mechanism of Jiedu Huoxue Decoction in Treating Type Ⅲ Prostatitis Is AsSociated with the ERK/p38 MAPK Signaling Pathway
Objective To study the mechanism of Jiedu Huoxue Decoction(JDHXD)in treating type Ⅲ prostatitis through ERK/p38 MAPK signaling pathway.Methods The rat model of chronic prostatitis was established by testectomy and using estradiol benzoate(EB).Thirty-six SD rats were randomly divided into sham(normal saline,4 mL·kg-1),model(normal saline,4 mL·kg-1),positive drug control(Qianliekang tablet suspension,4 mL·kg-1),and JDHXD low(4mL·kg-1),medium(8mL·kg-1)and high dose(12mL·kg-1)groups,with 6 rats in each group.All animals were administered continuously for 30 days.The histopathological changes of rats in each group were observed by hematoxylin-eosin(HE)staining.The expression levels of related inflammatory factors and proteins were detected by immunohistochemistry,RT-PCR and Western Blot.Results Compared to the sham group,rats in model group had chronic prostatitis with an increase in hyperplasia,edema,and inflammatory infiltration.However,JDHXD at different doses resulted in a reduction or disappearance of hyperplasia,edema,and inflammatory infiltration.The results of immunohistochemistry showed that the expression of p38,P-p38,STAT3 and P-STAT3 was elevated in the model group,but decreased in the positive drug control group and JDHXD groups.RT-PCR results showed that Qianliekang Tablets and JDHXD down-regulated the expression levels of TNF-α,IL-2,IL-6 and other cytokines in prostate tissue of rats at mRNA level.The results of Western Blot showed that p38,P-p38,P-ERK1/2,STAT3 and P-STAT3 were highly expressed in the process of disease progression in rats,while JDHXD down-regulated their expression,thereby inhibiting the excessive activation of related inflammatory factors.Conclusion JDHXD inhibits the excessive activation of ERK/p38 MAPK signaling pathway,thereby down-regulating the expression of IL-2,IL-6,TNF-α and other inflammatory factors,to improve the prostate tissue inflammatory infiltration and improve the inflammatory reaction.
Jiedu Huoxue Decoctiontype Ⅲ prostatitisERK/p38 MAPK pathwaytheory of poison and stasis
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