Determination of Taxifolin and Quercetin in Polygoni Orientalis Fructus by UPLC
Objective To apply ultra performance liquid chromatography(UPLC)to establish a method for the simultaneous determination of taxifolin and quercetin in Polygoni Orientalis Fructus.Methods The separation was performed on an Agilent ZORBAX SB-C18 column with the gradient elution of acetonitrile-0.1％phosphoric acid in water as the mobile phase,the column temperature was 25 ℃,the detection wavelength was 290 nm,the flow rate was 0.2 mL·min-1,and the injection volume was 1 μL.Results The.established analytical method has been examined and found to be satisfactory.Taxifolin and quercetin exhibited a good linear relationship within the concentration range of 3.258 to 104.272 µg·mL-1 and 2.983 to 95.452 μg·mL-1,respectively,with a correlation coefficient(r2=0.9999).The average recovery rates of the spiked samples were 98.8％and 98.8％,with relative standard deviation(RSD)values of 1.9％and 2.2％,respectively.Conclusion The method is simple,rapid,and can determine the contents of taxifolin and quercetin at the same wavelength,which can provide a reference basis for the quality control of Polygoni Orientalis Fructus.
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