UPLC法测定水红花子中花旗松素及槲皮素的含量
Determination of Taxifolin and Quercetin in Polygoni Orientalis Fructus by UPLC
刘玉甜 1黎冠兰 1韩丽娟 1钱正明 2李宁3
作者信息
- 1. 康美华大基因技术有限公司,广东深圳 518128;康美药业股份有限公司,广东揭阳 515300
- 2. 湘南学院医学影像检验与康复学院,湖南郴州 423000
- 3. 康美华大基因技术有限公司,广东深圳 518128;康美药业股份有限公司,广东揭阳 515300;云南中医药大学中药学院,云南昆明 650500
- 折叠
摘要
目的 应用超高效液相色谱法(UPLC)建立同时测定水红花子药材中花旗松素和槲皮素含量的方法.方法 采用Agilent ZORBAX SB-C18色谱柱,以乙腈-0.1％磷酸水为流动相梯度洗脱,柱温25 ℃,检测波长290 nm,流速0.2 mL·min-1,进样量1 μL.结果 建立的分析方法方法学考察良好.花旗松素、槲皮素进样浓度分别在 3.258～104.272 μg·mL-1、2.983～95.452 μg·mL-1内呈良好的线性关系(r2=0.999 9),平均加样回收率为 98.8％、98.8％,RSD值为1.9％、2.2％.结论 本方法简便、快速,可在同一波长下快速测定水红花子中花旗松素及槲皮素的含量,为水红花子药材质量控制提供参考依据.
Abstract
Objective To apply ultra performance liquid chromatography(UPLC)to establish a method for the simultaneous determination of taxifolin and quercetin in Polygoni Orientalis Fructus.Methods The separation was performed on an Agilent ZORBAX SB-C18 column with the gradient elution of acetonitrile-0.1％phosphoric acid in water as the mobile phase,the column temperature was 25 ℃,the detection wavelength was 290 nm,the flow rate was 0.2 mL·min-1,and the injection volume was 1 μL.Results The.established analytical method has been examined and found to be satisfactory.Taxifolin and quercetin exhibited a good linear relationship within the concentration range of 3.258 to 104.272 µg·mL-1 and 2.983 to 95.452 μg·mL-1,respectively,with a correlation coefficient(r2=0.9999).The average recovery rates of the spiked samples were 98.8％and 98.8％,with relative standard deviation(RSD)values of 1.9％and 2.2％,respectively.Conclusion The method is simple,rapid,and can determine the contents of taxifolin and quercetin at the same wavelength,which can provide a reference basis for the quality control of Polygoni Orientalis Fructus.
关键词
水红花子/UPLC/花旗松素/槲皮素Key words
Polygoni Orientalis Fructus/UPLC/taxifolin/quercetin引用本文复制引用
基金项目
广东省药品监督管理局科技创新项目(2021ZDB05)
出版年
2024
NETL
NSTL
万方数据