首页|白果仁35％乙醇-水洗脱部位多糖及多肽的含量测定

白果仁35％乙醇-水洗脱部位多糖及多肽的含量测定

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目的 对白果仁止咳有效部位35％乙醇-水洗脱部位中多糖及多肽的含量进行测定,为白果仁的综合开发和质量控制提供科学依据.方法 采用苯酚-硫酸法、双缩脲法,建立标准曲线,并进行精密度、稳定性、重复性、加样回收率的考察.结果 白果仁35％乙醇-水洗脱部位中多糖和多肽均在浓度范围内有良好的线性关系,方法学验证RSD值均小于2％,加样回收率分别为97.96％、97.94％,多糖含量为13.99％,多肽含量为31.41％.结论 本研究建立的白果仁35％乙醇-水洗脱部位多糖和多肽含量测定方法简单可行,稳定性和重现性好.
Determination of Polysaccharide and Polypeptide Content in 35％Ethanol-Water Elution Fraction of Ginkgo Kernels
Objective To determine the content of polysaccharides and polypeptides in the 35％ethanol-water elution part of the effective part of ginkgo kernels,and to provide scientific basis for the comprehensive development and quality control of ginkgo kernels.Methods The standard curve was established by the phenol-sulfuric acid method and biuret method,and the precision,stability,repeatability,and recovery rate were investigated.Results The polysaccharides and polypeptides in the 35％ethanol-water elution part of ginkgo kernel had a good linear relationship in the concentration range.The RSD values of methodological verification were less than 2％.The recovery rates were 97.96％and 97.94％.The polysaccharides content was 13.99％,and the polypeptides content was 31.41％.Conclusion The established method for the determination of polypeptides and polysaccharides in the 35％ethanol-water elution part of ginkgo kernels is simple and feasible,with good stability and reproducibility.

the 35％ethanol-water elution part of ginkgo kernelspolysaccharidepolypeptidecontent determinationultraviolet spectrophotometry

夏应婷、王文君、王蕾、刘安、程锦堂、李国栋

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云南中医药大学中药学院,云南昆明 650500

中国中医科学院中药研究所道地药材品质保障与资源持续利用全国重点实验室,北京 100700

淄博市市级机关医院,山东淄博 255000

白果仁35％乙醇-水洗脱部位 多糖 多肽 含量测定 紫外分光光度法

国家自然科学基金项目中国中医科学院科技创新工程项目中国中医科学院科技创新工程项目中央公益性科研院所基本科研业务费专项中央公益性科研院所基本科研业务费专项中央公益性科研院所基本科研业务费专项

82374022CI2023E002CI2021A04409ZZ13-YQ-061ZXKT22012ZXKT22039

2024

10.19288/j.cnki.issn.1000-2723.2024.03.005

云南中医学院学报
云南中医学院

云南中医学院学报

影响因子：0.933
ISSN：1000-2723
年,卷(期)：2024.47(3)