首页|"柔筋温通"手法干预家兔骨骼肌慢性损伤的作用机制

"柔筋温通"手法干预家兔骨骼肌慢性损伤的作用机制

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
目的 探讨"柔筋温通"手法干预家兔骨骼肌慢性损伤的作用机制.方法 选用40只健康成年家兔,30只造模成功后分别设为实验组、对照组、模型组各10只,余下10只设为正常组.实验组采用"柔筋温通"手法干预,对照组采用地塞米松药物治疗,共治疗10次,模型组和正常组均正常喂养,治疗结束后取材检测,采用ELISA法检测TNF-α、IL-6的含量以及采用Western blot检测TGF-β1、CTGF的蛋白表达.结果 实验组兔骨骼肌中TNF-α、IL-6含量显著降低;CTGF、TGF-β1蛋白表达明显降低.结论 通过研究,"柔筋温通"手法通过降低骨骼肌损伤局部炎性因子(TNF-α、IL-6)的含量以及通过降低的TGF-β1、CTGF的蛋白表达,来抑制炎性粘连和炎症纤维组织增生,促进骨骼肌慢性损伤的修复.
The Mechanism of"Softening Tendons and Warming Tong"Manipulation to Intervene Chronic Skeletal Muscle Injury in Rabbits
Objective To explore the mechanism of"softening tendons and warming Tong"manipulation to intervene chronic skeletal muscle injury in rabbits.Methods 40 healthy adult rabbits were selected,30 rabbits were set as experimental group,control group and model group,and the remaining 10 rabbits were set as normal group.The experimental group used"soft tendons warming Tong"manipulation intervention,the control group used dexamethasone drug treatment,co-treatment 10 times,the model group and the normal group were fed normally.After treatment,samples were collected for detection,and TNF-α and IL-6 were detected by ELISA,and the protein expression of TGF-β1 and CTGF were detected by Western blot.Results The contents of TNF-α and IL-6 in skeletal muscle were significantly decreased.The protein expressions of CTGF and TGF-β1 were significantly decreased.Conclusion Through the study,"softening tendons and warming Tong"manipulation can reduce the content of local inflammatory factors(TNF-α,IL-6)in skeletal muscle injury as well as reduce the content of inflammatory factors TGF-β1,CTGF protein expression,to inhibit inflammatory adhesion and inflammatory fibrous tissue hyperplasia,promote skeletal muscle chronic damage repair of injury.

"softening tendons and warming Tong"manipulationchronic skeletal muscle injurymechanism of action

向勇、王春林、田启东、李蕊蕊、董有康

展开 >

云南中医药大学,云南昆明 650500

云南省中医医院,云南昆明 650021

"柔筋温通"手法 慢性骨骼肌损伤 作用机制

国家自然科学基金地区基金项目云南省科技厅中医联合专项-面上项目云南省卫生科技计划项目云南中医药大学校院联合基金项目云南省2020年高层次中医药人才学科带头人培养对象项目

81860884202001AZ070001-0222019FF002-0242018NS0024YXLH202107云卫中医发展发[2021]1号

2024

10.19288/j.cnki.issn.1000-2723.2024.04.005

云南中医学院学报
云南中医学院

云南中医学院学报

影响因子：0.933
ISSN：1000-2723
年,卷(期)：2024.47(4)