Objective:The famous toxic traditional Chinese medicine,Gelsemium elegans,is rich in monoter-pene indole alkaloids(MIAs).Most of the TCM chemical studies on Gelsemium elegans have focused on its root and stem,and now the alkaloids are studied.Methods:The total alkaloids on the ground of Gelsemium elegans were isola-ted and purified by silica gel column chromatography,MCI column chromatography,high performance liquid chroma-tography(HPLC)and other separation techniques,and the structure identification methods such as nuclear magnetic resonance spectroscopy(NMR)and mass spectrometry(MS)were used to conduct the study.Results:The composi-tion and structure of the obtained monomer were determined.Five oxymonoterpene indole alkaloids were isolated and i-dentified as 19-xo-gelsenicine(1),14-hydroxy-19-oxogelsenicine(2),gelsedilam(3),Nb-methylgelsedil-am(4)and gelsemoxonine(5),respectively,and the antifungal activity of the total alkaloid extract and compounds 1 and 3 of Gelsemium elegans were evaluated.Conclusion:The finding shows the total alkaloid extract of Gelsemium ele-gans can inhibit the mycelial growth of phytophthora capsici by 34.80％at the concentration of 100 μg/mL.
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