Objective:To study the effect of Shenling Baizhu Powder on apoptosis,cell cycle and invasion of colo-rectal cancer via Linc01615/miR-491-5p.Methods:Groups were constructed by cell transfection and divided blank control group,Shenling Baizhu Powder group,Shenling Baizhu Powder group+siRNA Linc01615,Shenling Baizhu Pow-der group+oe Linc01615,Shenling Baizhu Powder group+miR-491-5p mimics,Shenling Baizhu Powder group+miR-491-5p inhibitor group,Shenling Baizhu Powder group+miR-491-5p mimics+oe Linc01615.All groups were added to the corresponding serum for intervention.After treatment with the corresponding serum,Annexin V-FITC/PI double staining flow cytometry was used to detect apoptosis and cell cycle distribution,and transwell assay was used to detect cell invasion.Results:Shenling Baishu Powder inhibited cell proliferation,invasion and G0/G1 phase of cell cycle,and promoted apoptosis,and the effect was more significant after the addition of siRNA Linc01615 and miR-491-5p mimics.The difference was statistically significant(P＜0.05).Conclusion:Shenling Baizhu Powder may inhibit the invasion of LoVo cells through Linc01615/miR-491-5p,promote apoptosis and block the cell cycle in G0/G1 phase.
关键词
参苓白术散/Linc01615/miR-491-5p/结直肠癌
Key words
Shenling Baizhu Powder/Linc01615/miR-491-5p/Colorectal Cancer
维普
万方数据