Objective:To study the therapeutic effect and mechanism of Sanbao Hutong Decoction(SBHTG)on allergic rhinitis(AR)model of qi deficiency of lung and spleen in SD young mice induced by OVA through the chan-ges of immune factors and protein expression levels.Methods:32 SPF SD rats aged 2-3 weeks were divided into blank group A,model group B,western medicine group C and Chinese medicine group D,8 rats each group.After 5 days of adaptive feeding,except for group A,the other groups were given systemic sensitization+local sensitization method for 21 days,drug intervention began on the 15th day,and the treatment was given 14 days later.The pathologi-cal morphology of nasal mucosa was evaluated by HE staining,the changes of rhinitis related cytokines were detected by ELISA,and the changes of inflammatory related proteins were detected by Western blot.Results:Compared with those of group A,the nasal symptom score,serum IL-4 and IgE contents were significantly increased in group B(P＜0.01),and significantly decreased after intervention with Sanbao Hutong Decoction and loratadine.INF-γ content in serum and Foxp3 protein expression in spleen were significantly decreased(P＜0.01),and were significantly in-creased after intervention with Sanbao Hutong Decoction and loratadine.Nasal symptom scores,serum IgE and IL-4 levels of the rats in groups C and D were significantly decreased(P＜0.01).Serum INF-γ content in groups C and D was significantly decreased(P＜0.01).The expression of Foxp3 protein in spleen tissues of groups C and D was significantly increased(P＜0.01).The nasal mucosal cilia in groups C and D were more orderly and continuous than those in the model group,and the epithelial edema,goblet cells and EOS infiltration were less severe than those in group B.Conclusion:It is concluded that Sanbao Hutong Decoction can effectively regulate the immune function of young mice with allergic rhinitis model,which may be related to up-regulating INF-γ level and Foxp3 protein ex-pression level,and down-regulating IL-4 and IgE level.
关键词
变应性鼻炎/三宝护童羹/Foxp3蛋白/γ干扰素/白细胞介素4/免疫球蛋白E
Key words
Allergic Rhinitis/Sanbao Hutong Decoction/Foxp3 Protein/γ Interferon/Interleukin4/Immuno-globulin E
维普
万方数据