地红方通过miR-133b/DUSP1/JNK通路干预糖尿病肾病氧化应激损伤
Study on the Intervention of Didong Decoction on Oxidative Stress Injury of Diabetic Nephropathy through miR-133b/DUSP1/JNK Pathway
林姿 1何卫东 1杜思哲1
作者信息
- 1. 福建中医药大学附属人民医院,福建福州 350004
- 折叠
摘要
目的 研究地红方对糖尿病肾病细胞模型氧化应激损伤的影响.方法 地红方含药血清制备,小鼠肾小球系膜细胞常规培养、传代.按以下分组:对照组(5.5mmol/L葡萄糖+正常血清)、高糖组(30mmol/L葡萄糖+正常血清)、地红方低剂量组(30mmol/L葡萄糖+5％地红方含药血清)、地红方中剂量组(30mmol/L葡萄糖+10％地红方含药血清)、地红方高剂量组(30mmol/L葡萄糖+20％地红方含药血清).采用实时荧光定量 PCR 检测 miR-133b、DUSP1 mRNA 表达.Western blotting 检测 DUSP1、p-JNK、Drp1、Fis1 蛋白表达.Elisa检测SOD、MDA表达.结果 与高糖组比较,地红方组miR-133b、MDA、p-JNK、Drp1、Fis1表达明显下降(P＜0.05),而DUSP1、SOD表达显著升高(P＜0.05).结论 地红方可通过miR-133b/DUSP1/JNK通路调控线粒体分裂改善糖尿病肾病氧化应激损伤.
Abstract
Objective:To study the effect of Dihong Decoction on oxidative stress injury of diabetic nephropathy cell model.Methods:The serum containing Dihong Decoction was prepared,and mouse mesangial cells were cultured and passed through routine.Groups were divided as follows,control group(5.5mmol/L glucose+normal serum),high glucose group(30mmol/L glucose+normal serum),low dose Dihong Decoction group(30mmol/L glucose+5％Dihong Decoction containing serum),medium dose Dihong Decoction group(30mmol/L glucose+10％Dihong De-coction containing serum),and high dose Dihong Decoction group(30mm ol/L glucose+20％Dihong Decoction containing serum).Real-time fluorescent quantitative PCR was used to detect the mRNA expression of miR-133b and DUSP1.Western blotting to detect the expression of DUSP1,p-JNK,Drp1 and Fis1 proteins.The expressions of SOD and MDA were detected by Elisa.Results:Compared with that of the high glucose group,the expressions of miR-133b,MDA,P-JNK,Drp1 and Fis1 in Dihong Decoction groups were significantly decreased(P＜0.05),while the expressions of DUSP1 and SOD were significantly increased(P＜0.05).Conclusion:Dihong Decoction can regu-late mitochondrial division through miR-133b/DUSP1/JNK pathway to improve oxidative stress injury in diabetic ne-phropathy.
关键词
地红方/糖尿病肾病/miR-133b/DUSP1/JNK/氧化应激Key words
Dihong Decoction/Diabetic Nephropathy/miR-133b/DUSP1/JNK/Oxidative Stress引用本文复制引用
基金项目
福建省自然科学基金(2021J01896)
福建省卫生健康青年科研课题(2021QNA054)
出版年
2024
NETL
NSTL
万方数据