Study on the Intervention of Didong Decoction on Oxidative Stress Injury of Diabetic Nephropathy through miR-133b/DUSP1/JNK Pathway
Objective:To study the effect of Dihong Decoction on oxidative stress injury of diabetic nephropathy cell model.Methods:The serum containing Dihong Decoction was prepared,and mouse mesangial cells were cultured and passed through routine.Groups were divided as follows,control group(5.5mmol/L glucose+normal serum),high glucose group(30mmol/L glucose+normal serum),low dose Dihong Decoction group(30mmol/L glucose+5％Dihong Decoction containing serum),medium dose Dihong Decoction group(30mmol/L glucose+10％Dihong De-coction containing serum),and high dose Dihong Decoction group(30mm ol/L glucose+20％Dihong Decoction containing serum).Real-time fluorescent quantitative PCR was used to detect the mRNA expression of miR-133b and DUSP1.Western blotting to detect the expression of DUSP1,p-JNK,Drp1 and Fis1 proteins.The expressions of SOD and MDA were detected by Elisa.Results:Compared with that of the high glucose group,the expressions of miR-133b,MDA,P-JNK,Drp1 and Fis1 in Dihong Decoction groups were significantly decreased(P＜0.05),while the expressions of DUSP1 and SOD were significantly increased(P＜0.05).Conclusion:Dihong Decoction can regu-late mitochondrial division through miR-133b/DUSP1/JNK pathway to improve oxidative stress injury in diabetic ne-phropathy.
NETL
NSTL
万方数据
