Effects of Anti-aging and Life-prolonging Prescription on the Expression of Learning and Memory and Apoptosis-related Factors Bcl-2 and Bax in Aging Rats
Objective:To study the effect of Anti-aging and Life-prolonging Prescription on the expression of apoptosis-related factors Bel-2 and Bax in aging rats,and to explore the possible mechanism of the prescription.Methods:40 healthy SD male rats were randomly divided into normal group,aging model group,TCM group and pi-racetam group.The normal group was not treated,and the aging model group,TCM group and piracetam group were injected with 500 mg/kg D-galactose subcutaneously daily on the neck and back for 30 days.After 30 days of treat-ment,Morris water maze was used to conduct behavioral tests on the rats,and Bel-2 mRNA and Bax mRNA expres-sion were detected by in situ hybridization.Results:Compared with the rats in the normal group,the rats in the mod-el group acted slowly,the escape latency increased(P＜0.05),the expression of Bel-2 mRNA decreased(P＜0.05),and the expression of Bax mRNA increased(P＜0.05).Compared with that of the model group,the activity of rats in the TCM group increased,the escape latency time decreased(P＜0.05),the expression of Bcl-2 mRNA increased(P＜0.05),and the expression of Bax mRNA decreased(P＜0.05).Conclusion:Anti-aging and Life-prolonging Prescription can improve the spatial learning and memory ability of aging rats,and its mechanism may be related to the up-regulation of the expression of anti-apoptotic factor Bc12 and the down-regulation of key pro-apoptotic factor Bax.
Anti-aging and Life-prolonging PrescriptionAgingIn Situ HybridizationBel-2/BaxRat
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