Content Determination of Aristolochic Acid Ⅰ and Aristolochic Acid Ⅱ in Fufang Weitong Capsules by UPLC-MS/MS
Objective To establish an UPLC-MS/MS method for simultaneous determination of aristolochic acid Ⅰ and aristolochic acid Ⅱ in fufang weitong capsules.Methods Agilent Poroshell SB-C18(100 mm×2.1 mm,2.7 µm)column was used for separation.The mobile phase consisted of acetonitrile and 0.1%formic acid(containing 5 mmol/L ammonium formate)with gradient elution.The flow rate was 0.3 Ml/min and the column temperature was 30℃.Electrospray positive ion source was used for mass spectrometry detection by multiple reaction monitoring mode.Results The liner range of calibration curve was 1.05-210 ng/Ml(r=0.999 6),1.00-200 ng/Ml(r=0.999 9)for aristolochic acid Ⅰ and aristolochic acid Ⅱ.The recovery of aristolochic acidⅠwas 95.90%with RSD of 2.11%,and the recovery of aristolochic acid Ⅱ was 93.22%with RSD of 1.17%.The limit of detection and quantitation for aristolochic acid Ⅰ were 0.3 pg and 0.1 pg,for aristolochic acid Ⅱ were 0.7 pg and 0.2 pg.Conclusion The method has good specificity,sensitivity and accuracy,and it is suitable for the content determination of aristolochic acid Ⅰ and aristolochic acid Ⅱ in fufang weitong capsules.
万方数据
维普
