To Study the Mechanism of Xiyanping in Treating Acute Bronchitis Based on Network Pharmacology
Objective To study the mechanism of xiyanping injection in treating acute bronchitis by using network pharmacology and molecular docking.Methods Screening the active ingredients of xiyanping injection through literature and TCMSP database,and predicting its target by SwissTargetPrediction.To screen acute bronchitis related targets by using DrugBank,GeneCards,and OMIM databases,and merging active ingredients and acute bronchitis targets to obtain their intersections,constructing a protein-protein in teraction network(PPI)by using a String database,and visualizing the PPI map by using Cytoscape 3.7.2 software.GO and KEGG enrichment analysis were conducted on drug-disease targets through the Metascape database to predict the mechanism of action,and molecular docking validation was conducted through the AutoDockTools platform.Results Six components of xiyanping intersect with a total of 34 targets in acute bronchitis.The mechanism involves human T-cell leukemia virus infection,cancer pathway,hepatitis B,kaposi sarcoma associated herpes virus infection,EB virus infection,human cytomegalovirus infection,etc.Molecular docking shows that the key compounds of xiyanping in the treatment of bronchitis,including 17-hydrogen-9-dehydroandrographolide-19 sodium sulfate,17-hydrogen-9-dehydroandrographolide,14-deoxy-11-oxoandrographolide,14-deoxy-12-methoxyandrographolide,14-deoxyandrographolide,and andrographolide,bind well to targets such as IL6,EGFR,CXCL8,ICAM1,MMP9,MAPK8,MAPK14,and ITGB2.Conclusion Through network pharmacology and molecular docking,it's suggested that xiyanping may exert its therapeutic effect on acute bronchitis through antiviral therapy,regulation of IL-17 signaling pathway,and TNF signaling pathway.
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