Study on Adulteration of Schisandrae Chinensis Fructus in Hugan Tablets Based on HPLC-DAD Method
Objective HPLC-DAD method for the determination of anwulignan was established to identify the adulterated schisandrae chinensis fructus with schisandrae sphenanthera fructus in hugan tablets.Methods The chromatography was performed on Agilent ZORBAX Eclipse XDB-C18 column(250 mm×4.6 mm,5 μm)with mobile phase methanol-water(70:30)at column temperature of 30℃and flow rate of 1.0 mL/min.The detection wavelength was 280 nm,the reference wavelength was 360 nm,and the injection volume was 10 μL.Results The linear relationship of anwulignan was good in the concentration range from 12.064 to 120.640 μg/mL,the average recovery rate was 98.80%and the RSD value was 1.07%.Conclusion The established method for the determination of anwulignan content has high sensitivity,specificity and reproducibility,and it can be used for the rapid identification of the adulterated schisandrae chinensis fructus with schisandrae sphenanthera fructus in hugan tablets.
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